PMID- 18163653
OWN - NLM
STAT- MEDLINE
DCOM- 20080402
LR  - 20080811
IS  - 0743-7463 (Print)
IS  - 0743-7463 (Linking)
VI  - 24
IP  - 3
DP  - 2008 Feb 5
TI  - Surface modification of poly(dimethylsiloxane) with a perfluorinated alkoxysilane 
      for selectivity toward fluorous tagged peptides.
PG  - 1080-6
LID - 10.1021/la702038t [doi]
AB  - Poly(dimethylsiloxane) (PDMS) and similar polymers have proved to be of 
      widespread interest for use in microfluidic and similar microanalytical devices. 
      Surface modification of PDMS is required to extend the range of applications for 
      devices made of this polymer, however. Here we report on the grafting of 
      perfluorooctyltriethoxysilane via hydrolysis onto an oxidized PDMS substrate in 
      order to form a fluorinated microchannel. Such a fluorinated device could be used 
      for separating fluorous tagged proteins or peptides, similar to that which has 
      been recently demonstrated in a capillary electrophoresis system or in an open 
      tubular capillary column. The modified polymer is characterized using chemical 
      force titrations, contact angle measurements, and X-ray photoelectron 
      spectroscopy (XPS). We also report on a novel means of performing electroosmotic 
      measurements on this material to determine the surface zeta potential. As might 
      be expected, contact angle and chemical force titration measurements indicate the 
      fluorinated surface to be highly hydrophobic. XPS indicates that fluorocarbon 
      groups segregate to the surface of the polymer over a period of days following 
      the initial surface modification, presumably driven by a lower surface free 
      energy. One of the most interesting results is the zeta potential measurements, 
      which show that significant surface charge can be maintained across a wide range 
      of pH on this modified polymer, sufficient to promote electroosmotic flow in a 
      microfluidic chip. Matrix-assisted time-of-flight mass spectrometry (MALDI-TOF 
      MS) measurements show that a fluorous-tagged peptide will selectively adsorb on 
      the fluorinated PDMS in aqueous solution, demonstrating that the fluorinated 
      polymer could be used in devices designed for the enrichment or enhanced 
      detection of fluorous-labeled proteins and peptides.
FAU - Wang, Dan
AU  - Wang D
AD  - Department of Chemistry, Queen's University, Kingston, Ontario, Canada K7L 3N6.
FAU - Goel, Vishya
AU  - Goel V
FAU - Oleschuk, Richard D
AU  - Oleschuk RD
FAU - Horton, J Hugh
AU  - Horton JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071229
PL  - United States
TA  - Langmuir
JT  - Langmuir : the ACS journal of surfaces and colloids
JID - 9882736
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Fluorocarbons)
RN  - 0 (Peptides)
RN  - 0 (Silanes)
RN  - 0 (Silicones)
RN  - 63148-62-9 (baysilon)
SB  - IM
EIN - Langmuir. 2008 Aug 5;24(15):8395. Goel, Vishya [added]
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Fluorocarbons/chemistry
MH  - Hydrogen-Ion Concentration
MH  - Microfluidic Analytical Techniques
MH  - Peptides/*chemistry
MH  - Silanes/chemistry
MH  - Silicones/*chemistry
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Spectrum Analysis
MH  - Surface Properties
MH  - X-Rays
EDAT- 2008/01/01 09:00
MHDA- 2008/04/03 09:00
CRDT- 2008/01/01 09:00
PHST- 2008/01/01 09:00 [pubmed]
PHST- 2008/04/03 09:00 [medline]
PHST- 2008/01/01 09:00 [entrez]
AID - 10.1021/la702038t [doi]
PST - ppublish
SO  - Langmuir. 2008 Feb 5;24(3):1080-6. doi: 10.1021/la702038t. Epub 2007 Dec 29.