PMID- 18164154
OWN - NLM
STAT- MEDLINE
DCOM- 20080501
LR  - 20181113
IS  - 0378-8741 (Print)
IS  - 0378-8741 (Linking)
VI  - 116
IP  - 1
DP  - 2008 Feb 28
TI  - Toll-like receptor ligand-induced activation of murine DC2.4 cells is attenuated 
      by Panax notoginseng.
PG  - 179-86
LID - 10.1016/j.jep.2007.11.019 [doi]
AB  - The medicinal herb, Panax notoginseng, has been used for thousands of years in 
      traditional Chinese medicine and possesses anti-inflammatory properties. 
      Dendritic cells (DCs) play a central role in the regulation of both inflammation 
      and adaptive immunity. The aim of this study was to investigate the potential for 
      notoginseng extracts to modulate Toll-like receptor (TLR) ligand-induced 
      activation of cultured DC2.4 cells. Following stimulation with LPS, CpG or 
      poly(I:C) and treatment with 0-50micorg/ml notoginseng extract for 24 h, DCs were 
      evaluated for various phenotypic and functional readouts. Notoginseng reduced the 
      LPS-, CpG- and poly(I:C)-induced production of TNF-alpha by DC2.4 cells. Also, 
      IL-6 production by notoginseng-treated cells stimulated with LPS and CpG but not 
      poly(I:C) was reduced when compared to controls. TLR ligand-induced CD40 
      expression was attenuated by notoginseng. In contrast, notoginseng decreased CD86 
      levels on DCs activated with LPS and poly(I:C) but not CpG. Inhibition of 
      TNF-alpha production was time-dependent in LPS-stimulated cells, occurring only 
      with pretreatment or concurrent treatment of notoginseng but not after delayed 
      addition of the herbal extract. Additionally, ginsenoside Rg1 more effectively 
      inhibited LPS-stimulated cytokine production by DC2.4 cells than ginsenoside Rb1. 
      Taken together, these results demonstrate that notoginseng inhibits the 
      production of specific inflammatory molecules and innate immune responsiveness by 
      DCs following TLR activation.
FAU - Rhule, Ava
AU  - Rhule A
AD  - Department of Biomedical and Pharmaceutical Sciences, University of Montana, 
      Missoula, MT 59812-1552, USA.
FAU - Rase, Benjamin
AU  - Rase B
FAU - Smith, Jerry R
AU  - Smith JR
FAU - Shepherd, David M
AU  - Shepherd DM
LA  - eng
GR  - P20 RR017670/RR/NCRR NIH HHS/United States
GR  - P20 RR017670-030012/RR/NCRR NIH HHS/United States
GR  - P20 RR 017670/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20071122
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Ginsenosides)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Plant Extracts)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (Transcription Factor RelA)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 7413S0WMH6 (ginsenoside Rb1)
RN  - O84C90HH2L (Poly I-C)
RN  - PJ788634QY (ginsenoside Rg1)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Dendritic Cells/*drug effects
MH  - Ginsenosides/pharmacology
MH  - Interleukin-6/biosynthesis
MH  - Lipopolysaccharides/pharmacology
MH  - Mice
MH  - *Panax notoginseng
MH  - Plant Extracts/*pharmacology
MH  - Poly I-C/pharmacology
MH  - Time Factors
MH  - Toll-Like Receptors/*physiology
MH  - Transcription Factor RelA/metabolism
MH  - Tumor Necrosis Factor-alpha/biosynthesis
PMC - PMC2265075
MID - NIHMS40472
EDAT- 2008/01/01 09:00
MHDA- 2008/05/02 09:00
PMCR- 2009/02/28
CRDT- 2008/01/01 09:00
PHST- 2007/06/22 00:00 [received]
PHST- 2007/10/17 00:00 [revised]
PHST- 2007/11/15 00:00 [accepted]
PHST- 2008/01/01 09:00 [pubmed]
PHST- 2008/05/02 09:00 [medline]
PHST- 2008/01/01 09:00 [entrez]
PHST- 2009/02/28 00:00 [pmc-release]
AID - S0378-8741(07)00613-7 [pii]
AID - 10.1016/j.jep.2007.11.019 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2008 Feb 28;116(1):179-86. doi: 10.1016/j.jep.2007.11.019. Epub 
      2007 Nov 22.