PMID- 18164590
OWN - NLM
STAT- MEDLINE
DCOM- 20080327
LR  - 20171116
IS  - 0898-6568 (Print)
IS  - 0898-6568 (Linking)
VI  - 20
IP  - 3
DP  - 2008 Mar
TI  - Tumor necrosis factor-alpha (TNF-alpha) induces integrin CD11b/CD18 (Mac-1) 
      up-regulation and migration to the CC chemokine CCL3 (MIP-1alpha) on human 
      neutrophils through defined signalling pathways.
PG  - 557-68
LID - 10.1016/j.cellsig.2007.11.008 [doi]
AB  - Strong evidence suggests that neutrophils may play an active role in acute and 
      chronic inflammatory disorders, such as rheumatoid arthritis and atherosclerosis. 
      Given the role of pro-inflammatory cytokine TNF-alpha in these inflammatory 
      processes, we planned the present study to investigate the effect of short term 
      incubation with TNF-alpha on neutrophil migration to CCL3, a chemokine produced 
      in inflammatory sites and normally devoid of neutrophil chemotactic properties. 
      We found that TNF-alpha primed neutrophils for migration to CCL3 via CCR5. 
      TNF-alpha-induced migration was a consequence of the TNF-alpha-induced 
      up-regulation of integrin CD11b/CD18 (Mac-1) on neutrophil surface. Furthermore, 
      TNF-alpha activity was found to be strictly dependent on the activation of ERK 
      1/2 p44, cooperating with the intracellular pathways involving Src kinases, 
      PI3K/Akt, p38 MAPK, well known as activated in response to classical 
      chemoattractants (CXCL8) or priming agents (GM-CSF). On the contrary, the effect 
      of TNF-alpha on neutrophil migration to CCL3 was not dependent on JNK 1/2. In 
      conclusion, the present report shows that TNF-alpha unveils a previously unknown 
      capacity of neutrophils to migrate to CCL3 through the intervention of Mac-1. 
      TNF-alpha regulates Mac-1 up-regulation through signalling pathways, involving 
      various kinases, but not JNK 1/2. Although highly speculative, ERK 1/2 p44 may 
      represent a selective target for the pharmacologic manipulation of 
      neutrophil-mediated adverse activities in TNF-alpha-mediated inflammatory states.
FAU - Montecucco, Fabrizio
AU  - Montecucco F
AD  - Laboratory of Phagocyte Physiopathology, First Clinic of Internal Medicine, 
      Department of Internal Medicine and Medical Specialties, University of Genoa 
      Medical School, Genoa, Italy. fabrizio.montecucco@medecine.unige.ch
FAU - Steffens, Sabine
AU  - Steffens S
FAU - Burger, Fabienne
AU  - Burger F
FAU - Da Costa, Ana
AU  - Da Costa A
FAU - Bianchi, Giordano
AU  - Bianchi G
FAU - Bertolotto, Maria
AU  - Bertolotto M
FAU - Mach, Francois
AU  - Mach F
FAU - Dallegri, Franco
AU  - Dallegri F
FAU - Ottonello, Luciano
AU  - Ottonello L
LA  - eng
PT  - Journal Article
DEP - 20071126
PL  - England
TA  - Cell Signal
JT  - Cellular signalling
JID - 8904683
RN  - 0 (CCL3 protein, human)
RN  - 0 (CD11b Antigen)
RN  - 0 (CD18 Antigens)
RN  - 0 (Chemokine CCL3)
RN  - 0 (ITGAM protein, human)
RN  - 0 (Macrophage-1 Antigen)
RN  - 0 (Receptors, CCR5)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.10.2 (src-Family Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Adult
MH  - CD11b Antigen/*metabolism
MH  - CD18 Antigens/*metabolism
MH  - Chemokine CCL3/*metabolism
MH  - *Chemotaxis, Leukocyte
MH  - Humans
MH  - Inflammation/immunology/metabolism
MH  - Macrophage-1 Antigen/*metabolism
MH  - Middle Aged
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3/metabolism
MH  - Neutrophil Activation
MH  - Neutrophils/enzymology/immunology/*metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Receptors, CCR5/metabolism
MH  - Recombinant Proteins/metabolism
MH  - *Signal Transduction
MH  - Tumor Necrosis Factor-alpha/*metabolism
MH  - Up-Regulation
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
MH  - src-Family Kinases/metabolism
EDAT- 2008/01/01 09:00
MHDA- 2008/03/28 09:00
CRDT- 2008/01/01 09:00
PHST- 2007/10/12 00:00 [received]
PHST- 2007/11/15 00:00 [revised]
PHST- 2007/11/16 00:00 [accepted]
PHST- 2008/01/01 09:00 [pubmed]
PHST- 2008/03/28 09:00 [medline]
PHST- 2008/01/01 09:00 [entrez]
AID - S0898-6568(07)00364-6 [pii]
AID - 10.1016/j.cellsig.2007.11.008 [doi]
PST - ppublish
SO  - Cell Signal. 2008 Mar;20(3):557-68. doi: 10.1016/j.cellsig.2007.11.008. Epub 2007 
      Nov 26.