PMID- 18165318
OWN - NLM
STAT- MEDLINE
DCOM- 20080205
LR  - 20211203
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 105
IP  - 1
DP  - 2008 Jan 8
TI  - Clathrin light chains function in mannose phosphate receptor trafficking via 
      regulation of actin assembly.
PG  - 168-73
LID - 10.1073/pnas.0707269105 [doi]
AB  - Clathrin-coated vesicles (CCVs) are major carriers for endocytic cargo and 
      mediate important intracellular trafficking events at the trans-Golgi network 
      (TGN) and endosomes. Whereas clathrin heavy chain provides the structural 
      backbone of the clathrin coat, the role of clathrin light chains (CLCs) is poorly 
      understood. We now demonstrate that CLCs are not required for clathrin-mediated 
      endocytosis but are critical for clathrin-mediated trafficking between the TGN 
      and the endosomal system. Specifically, CLC knockdown (KD) causes the 
      cation-independent mannose-6 phosphate receptor (CI-MPR) to cluster near the TGN 
      leading to a delay in processing of the lysosomal hydrolase cathepsin D. A 
      recently identified binding partner for CLCs is huntingtin-interacting protein 
      1-related (HIP1R), which is required for productive interactions of CCVs with the 
      actin cytoskeleton. CLC KD causes mislocalization of HIP1R and overassembly of 
      actin, which accumulates in patches around the clustered CI-MPR. A 
      dominant-negative CLC construct that disrupts HIP1R/CLC interactions causes 
      similar alterations in CI-MPR trafficking and actin assembly. Thus, in mammalian 
      cells CLCs function in intracellular membrane trafficking by acting as 
      recruitment proteins for HIP1R, enabling HIP1R to regulate actin assembly on 
      clathrin-coated structures.
FAU - Poupon, Viviane
AU  - Poupon V
AD  - Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill 
      University, Montreal, QC, Canada.
FAU - Girard, Martine
AU  - Girard M
FAU - Legendre-Guillemin, Valerie
AU  - Legendre-Guillemin V
FAU - Thomas, Sebastien
AU  - Thomas S
FAU - Bourbonniere, Lyne
AU  - Bourbonniere L
FAU - Philie, Jacynthe
AU  - Philie J
FAU - Bright, Nicholas A
AU  - Bright NA
FAU - McPherson, Peter S
AU  - McPherson PS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071228
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Actins)
RN  - 0 (CD8 Antigens)
RN  - 0 (Clathrin Light Chains)
RN  - 0 (HAP1 protein, human)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Mannose Receptor)
RN  - 0 (Mannose-Binding Lectins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, Cell Surface)
RN  - EC 3.4.23.5 (Cathepsin D)
SB  - IM
MH  - Actins/*chemistry
MH  - Animals
MH  - Biological Transport
MH  - CD8 Antigens/biosynthesis
MH  - COS Cells
MH  - Cathepsin D/chemistry/metabolism
MH  - Chlorocebus aethiops
MH  - Clathrin Light Chains/chemistry/*physiology
MH  - Endocytosis
MH  - Golgi Apparatus/metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Lectins, C-Type/*metabolism
MH  - Mannose Receptor
MH  - Mannose-Binding Lectins/*metabolism
MH  - Models, Biological
MH  - Nerve Tissue Proteins/chemistry
MH  - RNA, Small Interfering/metabolism
MH  - Receptors, Cell Surface/*metabolism
MH  - trans-Golgi Network
PMC - PMC2224180
COIS- The authors declare no conflict of interest.
EDAT- 2008/01/01 09:00
MHDA- 2008/02/06 09:00
PMCR- 2008/07/08
CRDT- 2008/01/01 09:00
PHST- 2008/01/01 09:00 [pubmed]
PHST- 2008/02/06 09:00 [medline]
PHST- 2008/01/01 09:00 [entrez]
PHST- 2008/07/08 00:00 [pmc-release]
AID - 0707269105 [pii]
AID - 8752 [pii]
AID - 10.1073/pnas.0707269105 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2008 Jan 8;105(1):168-73. doi: 10.1073/pnas.0707269105. 
      Epub 2007 Dec 28.