PMID- 18165875
OWN - NLM
STAT- MEDLINE
DCOM- 20080909
LR  - 20181113
IS  - 0095-3628 (Print)
IS  - 0095-3628 (Linking)
VI  - 56
IP  - 2
DP  - 2008 Aug
TI  - Methanobrevibacter phylotypes are the dominant methanogens in sheep from 
      Venezuela.
PG  - 390-4
LID - 10.1007/s00248-007-9351-x [doi]
AB  - Rumen methanogens in sheep from Venezuela were examined using 16S rRNA gene 
      libraries and denaturing gradient gel electrophoresis (DGGE) profiles prepared 
      from pooled and individual PCR products from the rumen contents from 10 animals. 
      A total of 104 clones were examined, revealing 14 different 16S rRNA gene 
      sequences or phylotypes. Of the 14 phylotypes, 13 (99 of 104 clones) belonged to 
      the genus Methanobrevibacter, indicating that the genus Methanobrevibacter is the 
      most dominant component of methanogen populations in sheep in Venezuela. The 
      largest group of clones (41 clones) was 97.9-98.5% similar to Methanobrevibacter 
      gottschalkii. Two sequences were identified as possible new species, one 
      belonging to the genus Methanobrevibacter and the other belonging to the genus 
      Methanobacterium. DGGE analysis of the rumen contents from individual animals 
      also revealed 14 different bands with a range of 4-9 bands per animal.
FAU - Wright, Andre-Denis G
AU  - Wright AD
AD  - CSIRO Livestock Industries, Centre for Environment and Life Sciences, WA 6913, 
      Australia. andre-denis.wright@csiro.au
FAU - Ma, Xuanli
AU  - Ma X
FAU - Obispo, Nestor E
AU  - Obispo NE
LA  - eng
SI  - GENBANK/DQ519576
SI  - GENBANK/DQ519577
SI  - GENBANK/DQ519578
SI  - GENBANK/DQ519579
SI  - GENBANK/DQ519580
SI  - GENBANK/DQ519581
SI  - GENBANK/DQ519582
SI  - GENBANK/DQ519583
SI  - GENBANK/DQ519584
SI  - GENBANK/DQ519585
SI  - GENBANK/DQ519586
SI  - GENBANK/DQ519587
SI  - GENBANK/DQ519588
SI  - GENBANK/DQ519589
SI  - GENBANK/DQ519590
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071229
PL  - United States
TA  - Microb Ecol
JT  - Microbial ecology
JID - 7500663
RN  - 0 (DNA, Archaeal)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - DNA, Archaeal/analysis
MH  - DNA, Ribosomal/analysis
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Female
MH  - Genes, rRNA
MH  - Male
MH  - Methanobacterium/classification/genetics/isolation & purification
MH  - Methanobrevibacter/*classification/genetics/*isolation & purification
MH  - Molecular Sequence Data
MH  - *Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Rumen/*microbiology
MH  - Sequence Analysis, DNA
MH  - Sheep/*microbiology
MH  - Venezuela
EDAT- 2008/01/01 09:00
MHDA- 2008/09/10 09:00
CRDT- 2008/01/01 09:00
PHST- 2007/07/31 00:00 [received]
PHST- 2007/11/22 00:00 [accepted]
PHST- 2007/11/14 00:00 [revised]
PHST- 2008/01/01 09:00 [pubmed]
PHST- 2008/09/10 09:00 [medline]
PHST- 2008/01/01 09:00 [entrez]
AID - 10.1007/s00248-007-9351-x [doi]
PST - ppublish
SO  - Microb Ecol. 2008 Aug;56(2):390-4. doi: 10.1007/s00248-007-9351-x. Epub 2007 Dec 
      29.