PMID- 18166486
OWN - NLM
STAT- MEDLINE
DCOM- 20080415
LR  - 20171116
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 41
IP  - 3
DP  - 2008 Mar
TI  - Induction of novel cytokines and chemokines by advanced glycation endproducts 
      determined with a cytometric bead array.
PG  - 198-203
LID - 10.1016/j.cyto.2007.11.012 [doi]
AB  - Advanced glycation endproducts (AGEs) accumulate on long-lived protein deposits, 
      e.g. those composed of beta(2)-microglobulin (in dialysis-related amyloidosis) or 
      beta-amyloid peptide (in Alzheimer's disease). When AGEs bind to the "receptor 
      for advanced glycation endproducts", they activate redox-sensitive transcription 
      factors such as NF-kappaB, and subsequently induce the expression of 
      pro-inflammatory cytokines such as IL-1, IL-6 and TNF-alpha. Using a cytokine 
      bead array, we have further analyzed the Bovine Serum Albumin (BSA)-AGE induced 
      expression of selected cytokines/chemokines in two murine cell lines, RAW 264.7 
      macrophages and N-11 microglia. Our study showed that monocyte chemoattractant 
      protein-1 (MCP-1) and tumor necrosis factor (TNF-alpha) were both released in a 
      time-dependent manner from both RAW 264.7 macrophages and N-11 microglia upon 
      stimulation with BSA-AGE or lipopolysaccharide (LPS), which was used as a 
      positive control. Interestingly, MCP-1 was also constitutively expressed by 
      unstimulated cells, although at a lower levels. Much higher levels of IL-6 were 
      secreted by RAW 264.7 macrophages than by N-11 microglia in response to both 
      stimuli. IL-12p70, interferon-gamma and the anti-inflammatory cytokine IL-10 were 
      not induced by either LPS or BSA-AGE. Our results indicate a very similar pattern 
      of chemokine and cytokine expression induced by such different ligands as AGEs 
      and LPS indicating similar or convergent downstream signaling pathways.
FAU - Berbaum, Katrin
AU  - Berbaum K
AD  - Department of Biochemistry and Molecular Biology & Comparative Genomics Centre, 
      School of Pharmacy and Molecular Sciences, James Cook University, Townsville, 
      Australia.
FAU - Shanmugam, Kirubakaran
AU  - Shanmugam K
FAU - Stuchbury, Grant
AU  - Stuchbury G
FAU - Wiede, Florian
AU  - Wiede F
FAU - Korner, Heiner
AU  - Korner H
FAU - Munch, Gerald
AU  - Munch G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071231
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Glycation End Products, Advanced)
RN  - 0 (Lipopolysaccharides)
RN  - 27432CM55Q (Serum Albumin, Bovine)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Line
MH  - Chemokines/analysis/*metabolism
MH  - Cytokines/analysis/*metabolism
MH  - Glycation End Products, Advanced/*metabolism/pharmacology
MH  - Lipopolysaccharides/metabolism/pharmacology
MH  - Macrophages/drug effects/metabolism
MH  - Mice
MH  - Microglia/drug effects/metabolism
MH  - Protein Array Analysis/methods
MH  - Serum Albumin, Bovine/pharmacology
EDAT- 2008/01/02 09:00
MHDA- 2008/04/16 09:00
CRDT- 2008/01/02 09:00
PHST- 2007/08/08 00:00 [received]
PHST- 2007/10/17 00:00 [revised]
PHST- 2007/11/23 00:00 [accepted]
PHST- 2008/01/02 09:00 [pubmed]
PHST- 2008/04/16 09:00 [medline]
PHST- 2008/01/02 09:00 [entrez]
AID - S1043-4666(07)00499-1 [pii]
AID - 10.1016/j.cyto.2007.11.012 [doi]
PST - ppublish
SO  - Cytokine. 2008 Mar;41(3):198-203. doi: 10.1016/j.cyto.2007.11.012. Epub 2007 Dec 
      31.