PMID- 18166836
OWN - NLM
STAT- MEDLINE
DCOM- 20080116
LR  - 20181201
IS  - 1556-1380 (Electronic)
IS  - 1556-0864 (Linking)
VI  - 3
IP  - 1
DP  - 2008 Jan
TI  - Comparison between epidermal growth factor receptor (EGFR) gene expression in 
      primary non-small cell lung cancer (NSCLC) and in fine-needle aspirates from 
      distant metastatic sites.
PG  - 18-22
LID - 10.1097/JTO.0b013e31815e8ba2 [doi]
AB  - PURPOSE: Epidermal growth factor receptor (EGFR) gene copy number obtained by 
      fluorescence in situ hybridization (FISH) has been recently found to predict 
      treatment outcome in non-small cell lung cancer (NSCLC) patients receiving EGFR 
      tyrosine kinase inhibitors. However, it is still unknown whether EGFR status 
      differs in metastases compared with primary NSCLC. In all studies FISH have been 
      performed on histologic material. The possibility to perform FISH analysis on 
      cytologic material obtained by fine-needle aspiration from superficial and 
      visceral metastases would allow us to know the real EGFR status avoiding invasive 
      diagnostic procedures. METHODS: EGFR gene copy number was analyzed by FISH on 
      fine-needle aspirates obtained from 31 patients with metastatic NSCLC and the 
      results were compared with those obtained on corresponding paraffin histologic 
      sections from the primary tumor. RESULTS: The feasibility of EGFR FISH on 
      cytology was 90% (28 of 31 patients). EGFR FISH was positive in 61% (17 of 28 
      patients) of the metastases and in 36% (10 of 28 patients) of the primary tumors. 
      Nine of the 28 cases (32%) were EGFR positive on both primary tumor and 
      metastatic site and 10 (36%) were negative on both primary tumor and metastasis. 
      Nine of the 28 cases (32%) showed discordance of primary tumor versus metastasis 
      (McNemar test; p = 0.041). CONCLUSIONS: EGFR FISH can be reliably assessed on 
      fine-needle aspirates obtained from NSCLC metastases. We found that EGFR gene 
      copy number is discordant between primary NSCLC and the corresponding distant 
      metastatic sites in a significant proportion of cases. These findings should be 
      considered in future studies designed to elucidate the predictive role of EGFR 
      FISH in NSCLC.
FAU - Bozzetti, Cecilia
AU  - Bozzetti C
AD  - Division of Medical Oncology, University Hospital of Parma, Parma, Italy.
FAU - Tiseo, Marcello
AU  - Tiseo M
FAU - Lagrasta, Costanza
AU  - Lagrasta C
FAU - Nizzoli, Rita
AU  - Nizzoli R
FAU - Guazzi, Annamaria
AU  - Guazzi A
FAU - Leonardi, Francesco
AU  - Leonardi F
FAU - Gasparro, Donatello
AU  - Gasparro D
FAU - Spiritelli, Elena
AU  - Spiritelli E
FAU - Rusca, Michele
AU  - Rusca M
FAU - Carbognani, Paolo
AU  - Carbognani P
FAU - Majori, Maria
AU  - Majori M
FAU - Franciosi, Vittorio
AU  - Franciosi V
FAU - Rindi, Guido
AU  - Rindi G
FAU - Ardizzoni, Andrea
AU  - Ardizzoni A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Thorac Oncol
JT  - Journal of thoracic oncology : official publication of the International 
      Association for the Study of Lung Cancer
JID - 101274235
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biopsy, Fine-Needle
MH  - Carcinoma, Non-Small-Cell Lung/*metabolism/*pathology/surgery
MH  - Carcinoma, Squamous Cell/metabolism/pathology/surgery
MH  - ErbB Receptors/genetics/*metabolism
MH  - Feasibility Studies
MH  - Female
MH  - Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/*metabolism/*pathology/surgery
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Metastasis
EDAT- 2008/01/02 09:00
MHDA- 2008/01/17 09:00
CRDT- 2008/01/02 09:00
PHST- 2008/01/02 09:00 [pubmed]
PHST- 2008/01/17 09:00 [medline]
PHST- 2008/01/02 09:00 [entrez]
AID - S1556-0864(15)31197-7 [pii]
AID - 10.1097/JTO.0b013e31815e8ba2 [doi]
PST - ppublish
SO  - J Thorac Oncol. 2008 Jan;3(1):18-22. doi: 10.1097/JTO.0b013e31815e8ba2.