PMID- 18167273
OWN - NLM
STAT- MEDLINE
DCOM- 20080630
LR  - 20081121
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 87
IP  - 39
DP  - 2007 Oct 23
TI  - [Allogeneic dendritic cell vaccine pulsed with heat shocked tumor cell lysate can 
      enhance antitumor immunity].
PG  - 2785-90
AB  - OBJECTIVE: To explore whether the allogeneic dendritic cells-pulsed with heat 
      shock protein (HSP)-70-rich tumor cell lysate can enhance the antitumor immunity. 
      METHODS: Mouse Lewis lung cancer cells were dipped into water of the temperature 
      of 43 degrees C for 1 h so as to undergo heat shock. C57BLK(b)) to elicit 
      increased hsp70 expression, The LLCs were lysed by freeze thawing and the 
      supernatant was collected (LhTCL). Dendritic cells (DCs) were isolated from 
      allogeneic inbred mice (615Kk or BALB/CKd) bone marrow (Bm-DCs), and were pulsed 
      with the LhTCL that underwent heart shock. The phenotypes of the Bm-DCs were 
      analyzed by flow cytometry (FCM). The level of IL-12 was detected by ELISA. The 
      Bm-Dcs of Balb/c mice that were pulsed by LhTCL or not, were injected into the 
      foot pads of C57BL mice as vaccine, 3 d later the lymph nodes in the drainage 
      area of popliteal fossa were taken out to make single cell suspension to undergo 
      immunohistochemistry. Inducible hsp70 in LhTCL was assayed by Western Blotting. 
      The ability of taking up LhTCL by immature DC was evaluated by phagocytosis 
      experiment in vitro and the uptake of CFSE labeled allogeneic DC by recipient DC 
      was evaluated by tracing experiment in vivo. The supernatant cytokine release was 
      determined by sandwich ELISA. Double stained DC was confirmed by FCM and confocal 
      microscopy. Cytotoxic activity was assessed by LDH release assay. The 
      immuno-effect of vaccine was valued by immunoprophylaxis in LLC transplanted 
      model and therapy experiment of LLC-bearing mice of early stage. RESULT: 43 
      degrees C heating significantly increased the expression of inducible hsp70 in 
      the LLCs. The allogeneic immature DC s showed full ability to uptake LhTCL and 
      enhanced the expression of CD86 molecules on DC surface and IL-12 secretion, 
      showing that they promoted the maturation of DCs. Subcutaneous injection of 
      allogeneic DC/LhTCL promoted the uptake of allogeneic DC by recipient DC. The 
      spleen cells from the C57BL mice immunized with allogeneic DC/LhTCL vaccine 
      specifically killed the viable LLCs, and secreted Th-1 related cytokine (i.e. 
      high level of INF-gamma and low level of IL-4/IL-10). The experiment of 
      immunoprophylaxis of LLC transplant and therapy of LLC-bearing C57BL mice both 
      confirmed that immunization with allogeneic DC/LhTCL prevented or delayed the 
      growth of LLCs, which elicited higher immunological effect than that with 
      syngeneic DCs/HLTCL. CONCLUSION: Immunization with vaccine of allogeneic DC 
      pulsed with heat shocked hsp70-rich tumor cell lysate is able to enhance 
      antitumor immunity.
FAU - You, Jian
AU  - You J
AD  - Department of Thoracic Surgery, Tianjin Medical University Cancer Hospital & 
      Institute, Tianjin 300060, China.
FAU - Wang, Chang-li
AU  - Wang CL
FAU - Hao, Xi-shan
AU  - Hao XS
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Cancer Vaccines)
RN  - 0 (HSC70 Heat-Shock Proteins)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Adoptive Transfer/*methods
MH  - Animals
MH  - Blotting, Western
MH  - Cancer Vaccines/genetics/immunology/*therapeutic use
MH  - Carcinoma, Lewis Lung/metabolism/pathology/*therapy
MH  - Cell Line, Tumor
MH  - Dendritic Cells/*immunology/transplantation
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Flow Cytometry
MH  - HSC70 Heat-Shock Proteins/metabolism
MH  - Hot Temperature
MH  - Immunohistochemistry
MH  - Interleukin-12/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Survival Analysis
EDAT- 2008/01/03 09:00
MHDA- 2008/07/01 09:00
CRDT- 2008/01/03 09:00
PHST- 2008/01/03 09:00 [pubmed]
PHST- 2008/07/01 09:00 [medline]
PHST- 2008/01/03 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2007 Oct 23;87(39):2785-90.