PMID- 18175933
OWN - NLM
STAT- MEDLINE
DCOM- 20080206
LR  - 20190720
IS  - 0918-6158 (Print)
IS  - 0918-6158 (Linking)
VI  - 31
IP  - 1
DP  - 2008 Jan
TI  - Biochemical characterization of transporter associated with antigen processing 
      (TAP)-like (ABCB9) expressed in insect cells.
PG  - 1-5
AB  - The ATP-binding cassette (ABC) transporter, transporter associated with antigen 
      processing (TAP)-like (TAPL) tagged with a histidine cluster was overexpressed, 
      amounting to as much as 1-2% of total membrane proteins in Drosophila cell line 
      S2. TAPL was effectively solubilized from membranes by Triton X-100, NP-40 and 
      n-dodecyl-beta-D-maltoside. Solubilized TAPL bound ATP-agarose and adenosine 
      5'-diphosphate (ADP)-agarose but not adenosine 5'-monophosphate (AMP)-agarose. 
      The binding was competed for by excess free ATP, ADP, guanosine 5'-triphosphate 
      (GTP) and dATP but not by AMP. Pyrimidine nucleotides such as uridine 
      5'-triphosphate (UTP) and cytidine 5'-triphosphate (CTP) were less effective 
      competitors, suggesting that purine nucleotide triphosphates are substrates for 
      TAPL. The ATP-binding of TAPL required Mg(2+), and was observed at neutral pH. 
      Chemical cross-linking experiments suggested that TAPL forms a homodimer in the 
      membrane and under the solubilized conditions.
FAU - Ohara, Tomomi
AU  - Ohara T
AD  - Laboratory of Biochemistry and Molecular Biology, Graduate School of 
      Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan.
FAU - Ohashi-Kobayashi, Ayako
AU  - Ohashi-Kobayashi A
FAU - Maeda, Masatomo
AU  - Maeda M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (ABCB9 protein, human)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Recombinant Proteins)
RN  - 61D2G4IYVH (Adenosine Diphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - ATP-Binding Cassette Transporters/chemistry/*metabolism
MH  - Adenosine Diphosphate/metabolism
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Cell Line
MH  - Dimerization
MH  - Drosophila
MH  - Recombinant Proteins/metabolism
MH  - Solubility
EDAT- 2008/01/08 09:00
MHDA- 2008/02/07 09:00
CRDT- 2008/01/08 09:00
PHST- 2008/01/08 09:00 [pubmed]
PHST- 2008/02/07 09:00 [medline]
PHST- 2008/01/08 09:00 [entrez]
AID - JST.JSTAGE/bpb/31.1 [pii]
AID - 10.1248/bpb.31.1 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2008 Jan;31(1):1-5. doi: 10.1248/bpb.31.1.