PMID- 1817642
OWN - NLM
STAT- MEDLINE
DCOM- 19920714
LR  - 20120531
IS  - 0914-7470 (Print)
IS  - 0914-7470 (Linking)
VI  - 4
IP  - 4
DP  - 1991 Dec
TI  - [Advances in molecular genetic diagnosis of leukemia].
PG  - 279-84
AB  - Recently, Molecular genetics has remarkably advanced and it is introduced in 
      medicine. The use of recombinant DNA methods for the diagnosis of leukemias is 
      reported with special reference to the contribution of cytogenetic findings, such 
      as specific chromosome aberrations previously obtained. Therefore, cytogenetic 
      studies on Ph1 chromosome and other specific aberrations found in leukemias are 
      historically reviewed. Using Southern blotting, PFGE, PCR, and in situ chromosome 
      mapping techniques we have analyzed many cases with CML and cases with ALL. We 
      found M-bcr rearrangements not only in standard Ph1, but also in complex types 
      and in Ph1 (-) ve CML. Chromosomal in situ hybridization was very informative 
      identifying transposition of bcr and abl genes between chromosomes 22 and 9. In 
      this connection, FISH (fluorescence in situ hybridization) technique was 
      developed by us, which is expected to have an exceptional power of analysis. ALL 
      had either M-bcr or m-bcr rearrangements, the latter being identified by PFGE. 
      Next, application of PCR technique that enables to obtain more than 10(5) copies 
      of target sequences could monitor minimal residual diseases in CML. Recently, the 
      relevant gene were cloned respectively in FAB-M2 and APL (FAB-M3), so that 
      detection of minimal residual diseases will be successfully performed in these 
      types of leukemia. Finally, targeting chemotherapy using antisense sequences is 
      prospectively described.
FAU - Abe, T
AU  - Abe T
AD  - Department of Hygiene, Kyoto Prefectural University of Medicine.
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PT  - Review
PL  - Japan
TA  - Hum Cell
JT  - Human cell
JID - 8912329
SB  - IM
MH  - Blotting, Southern
MH  - Chromosome Aberrations
MH  - Chromosome Mapping
MH  - Gene Rearrangement
MH  - Humans
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*diagnosis
MH  - Philadelphia Chromosome
RF  - 25
EDAT- 1991/12/01 00:00
MHDA- 1991/12/01 00:01
CRDT- 1991/12/01 00:00
PHST- 1991/12/01 00:00 [pubmed]
PHST- 1991/12/01 00:01 [medline]
PHST- 1991/12/01 00:00 [entrez]
PST - ppublish
SO  - Hum Cell. 1991 Dec;4(4):279-84.