PMID- 18178870
OWN - NLM
STAT- MEDLINE
DCOM- 20080415
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 111
IP  - 5
DP  - 2008 Mar 1
TI  - Organ-derived dendritic cells have differential effects on alloreactive T cells.
PG  - 2929-40
LID - 10.1182/blood-2007-06-096602 [doi]
AB  - Dendritic cells (DCs) are considered critical for the induction of 
      graft-versus-host disease (GVHD) after bone marrow transplantation (BMT). In 
      addition to their priming function, dendritic cells have been shown to induce 
      organ-tropism through induction of specific homing molecules on T cells. Using 
      adoptive transfer of CFSE-labeled cells, we first demonstrated that alloreactive 
      T cells differentially up-regulate specific homing molecules in vivo. Host-type 
      dendritic cells from the GVHD target organs liver and spleen or skin- and 
      gut-draining lymph nodes effectively primed naive allogeneic T cells in vitro 
      with the exception of liver-derived dendritic cells, which showed less 
      stimulatory capacity. Gut-derived dendritic cells induced alloreactive donor T 
      cells with a gut-homing phenotype that caused increased GVHD mortality and 
      morbidity compared with T cells stimulated with dendritic cells from spleen, 
      liver, and peripheral lymph nodes in an MHC-mismatched murine BMT model. However, 
      in vivo analysis demonstrated that the in vitro imprinting of homing molecules on 
      alloreactive T cells was only transient. In conclusion, organ-derived dendritic 
      cells can efficiently induce specific homing molecules on alloreactive T cells. A 
      gut-homing phenotype correlates with increased GVHD mortality and morbidity after 
      murine BMT, underlining the importance of the gut in the pathophysiology of GVHD.
FAU - Kim, Theo D
AU  - Kim TD
AD  - Department of Medicine and Immunology, Memorial Sloan-Kettering Cancer Center, 
      1275 York Avenue, New York, NY 10021, USA.
FAU - Terwey, Theis H
AU  - Terwey TH
FAU - Zakrzewski, Johannes L
AU  - Zakrzewski JL
FAU - Suh, David
AU  - Suh D
FAU - Kochman, Adam A
AU  - Kochman AA
FAU - Chen, Megan E
AU  - Chen ME
FAU - King, Chris G
AU  - King CG
FAU - Borsotti, Chiara
AU  - Borsotti C
FAU - Grubin, Jeremy
AU  - Grubin J
FAU - Smith, Odette M
AU  - Smith OM
FAU - Heller, Glenn
AU  - Heller G
FAU - Liu, Chen
AU  - Liu C
FAU - Murphy, George F
AU  - Murphy GF
FAU - Alpdogan, Onder
AU  - Alpdogan O
FAU - van den Brink, Marcel R M
AU  - van den Brink MR
LA  - eng
GR  - P20 CA 103694/CA/NCI NIH HHS/United States
GR  - CA 107096/CA/NCI NIH HHS/United States
GR  - R01 HL069929/HL/NHLBI NIH HHS/United States
GR  - R01 CA107096/CA/NCI NIH HHS/United States
GR  - P01 CA023766/CA/NCI NIH HHS/United States
GR  - CA 33049/CA/NCI NIH HHS/United States
GR  - P01 CA033049/CA/NCI NIH HHS/United States
GR  - P30 AR042689/AR/NIAMS NIH HHS/United States
GR  - P30 CA008748/CA/NCI NIH HHS/United States
GR  - CA 023766/CA/NCI NIH HHS/United States
GR  - R01 HL084815/HL/NHLBI NIH HHS/United States
GR  - P30 AR042689-159006/AR/NIAMS NIH HHS/United States
GR  - P20 CA103694/CA/NCI NIH HHS/United States
GR  - R01 HL084815-17/HL/NHLBI NIH HHS/United States
GR  - HL 69929/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080104
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Integrins)
RN  - 0 (Isoantigens)
RN  - 0 (Ligands)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptors, Lymphocyte Homing)
RN  - 0 (Selectins)
RN  - 0 (integrin alpha4beta7)
SB  - IM
MH  - Animals
MH  - Cell Proliferation/drug effects
MH  - Dendritic Cells/*cytology/drug effects/*immunology
MH  - Gene Expression Profiling
MH  - Graft vs Host Disease
MH  - Humans
MH  - Integrins/metabolism
MH  - Isoantigens/*immunology
MH  - Ligands
MH  - Lipopolysaccharides/pharmacology
MH  - Liver/cytology/drug effects
MH  - Lymphocyte Activation/drug effects
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Oligonucleotide Array Sequence Analysis
MH  - Organ Specificity/drug effects
MH  - Phenotype
MH  - Receptors, Lymphocyte Homing/genetics/metabolism
MH  - Selectins/metabolism
MH  - Survival Rate
MH  - T-Lymphocytes/cytology/drug effects/*immunology
MH  - Up-Regulation/drug effects/genetics
PMC - PMC2254543
EDAT- 2008/01/08 09:00
MHDA- 2008/04/16 09:00
PMCR- 2009/03/01
CRDT- 2008/01/08 09:00
PHST- 2008/01/08 09:00 [pubmed]
PHST- 2008/04/16 09:00 [medline]
PHST- 2008/01/08 09:00 [entrez]
PHST- 2009/03/01 00:00 [pmc-release]
AID - S0006-4971(20)51576-0 [pii]
AID - 2007/096602 [pii]
AID - 10.1182/blood-2007-06-096602 [doi]
PST - ppublish
SO  - Blood. 2008 Mar 1;111(5):2929-40. doi: 10.1182/blood-2007-06-096602. Epub 2008 
      Jan 4.