PMID- 18179646
OWN - NLM
STAT- MEDLINE
DCOM- 20080502
LR  - 20111117
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 71
IP  - 3
DP  - 2008 Mar
TI  - DNA typing by microbead arrays and PCR-SSP: apparent false-negative or -positive 
      hybridization or amplification signals disclose new HLA-B and -DRB1 alleles.
PG  - 238-41
LID - 10.1111/j.1399-0039.2007.00993.x [doi]
AB  - Human leukocyte antigen (HLA) typing by polymerase chain 
      reaction-sequence-specific oligonucleotide (PCR-SSO) hybridization on solid phase 
      (microbead assay) or polymerase chain reaction-sequence-specific primers 
      (PCR-SSP) requires interpretation softwares to detect all possible allele 
      combinations. These programs propose allele calls by taking into account 
      false-positive or false-negative signal(s). The laboratory has the option to 
      validate typing results in the presence of strongly cross-reacting or apparent 
      false-negative signals. Alternatively, these seemingly aberrant signals may 
      disclose novel variants. We report here four new HLA-B (B*5620 and B*5716) and 
      HLA-DRB1 alleles (DRB1*110107 and DRB1*1474) that were detected by apparent 
      false-negative or -positive hybridization or amplification patterns, and 
      ultimately resolved by sequencing. To avoid allele misassignments, a 
      comprehensive evaluation of acquired data as documented in a quality assurance 
      system is therefore required to confirm unambiguous typing interpretation.
FAU - Rahal, M
AU  - Rahal M
AD  - Transplantation Immunology Unit/LNRH, University Hospital Geneva, Geneva, 
      Switzerland.
FAU - Kervaire, B
AU  - Kervaire B
FAU - Villard, J
AU  - Villard J
FAU - Tiercy, J-M
AU  - Tiercy JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080107
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (DNA Primers)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Alleles
MH  - Base Sequence
MH  - DNA/*genetics
MH  - DNA Fingerprinting/*methods/statistics & numerical data
MH  - DNA Primers/genetics
MH  - False Negative Reactions
MH  - False Positive Reactions
MH  - HLA-B Antigens/*genetics
MH  - HLA-DR Antigens/*genetics
MH  - HLA-DRB1 Chains
MH  - Histocompatibility Testing/*methods/statistics & numerical data
MH  - Humans
MH  - Microspheres
MH  - Molecular Sequence Data
MH  - Nucleic Acid Amplification Techniques
MH  - Nucleic Acid Hybridization
MH  - Polymerase Chain Reaction/methods
MH  - Sequence Homology, Nucleic Acid
EDAT- 2008/01/09 09:00
MHDA- 2008/05/03 09:00
CRDT- 2008/01/09 09:00
PHST- 2008/01/09 09:00 [pubmed]
PHST- 2008/05/03 09:00 [medline]
PHST- 2008/01/09 09:00 [entrez]
AID - TAN993 [pii]
AID - 10.1111/j.1399-0039.2007.00993.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2008 Mar;71(3):238-41. doi: 10.1111/j.1399-0039.2007.00993.x. 
      Epub 2008 Jan 7.