PMID- 18179856
OWN - NLM
STAT- MEDLINE
DCOM- 20080905
LR  - 20220309
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 36
IP  - 3
DP  - 2008 Mar
TI  - Derivation and immunological characterization of mesenchymal stromal cells from 
      human embryonic stem cells.
PG  - 350-9
LID - 10.1016/j.exphem.2007.10.007 [doi]
AB  - OBJECTIVE: We have previously shown the simultaneous generation of CD73(+) 
      mesenchymal stromal cells (MSCs) along with CD34(+) hematopoietic cells from 
      human embryonic stem cells (ESCs) when they are cocultured with OP9 murine 
      stromal cells. We investigated whether MSCs can be derived from human ESCs 
      without coculturing with OP9 cells, and if such cells exhibit immunological 
      properties similar to MSCs derived from adult human bone marrow (BM). MATERIALS 
      AND METHODS: Our starting populations were undifferentiated human ESCs cultured 
      on Matrigel-coated plates without feeder cells. The differentiated 
      fibroblast-looking cells were tested for expression of MSC markers and their 
      potential for multilineage differentiation. We investigated surface expression of 
      human leukocyte antigen (HLA) molecules on these MSCs before and after treatment 
      with interferon-gamma (IFN-gamma). We also tested the proliferative response of 
      T-lymphocytes toward MSCs and the effects of MSCs in mixed lymphocyte reaction 
      (MLR) assays. RESULTS: We derived populations of MSCs from human ESCs with 
      morphology, cell surface marker characteristics, and differentiation potential 
      similar to adult BM-derived MSCs. Similar to BM-derived MSCs, human ESC-derived 
      MSCs express cell surface HLA class I (HLA-ABC) but not HLA class II (HLA-DR) 
      molecules. However, stimulation with IFN-gamma induced the expression of HLD-DR 
      molecules. Human ESC-derived MSCs did not induce proliferation of T-lymphocytes 
      when cocultured with peripheral blood mononuclear cells. Furthermore, ESC-derived 
      MSCs suppressed proliferation of responder T-lymphocytes in MLR assays. 
      CONCLUSIONS: MSCs can be derived from human ESCs without feeder cells. These 
      human ESC-derived MSCs have cell surface markers, differentiation potentials, and 
      immunological properties in vitro that are similar to adult BM-derived MSCs.
FAU - Trivedi, Parul
AU  - Trivedi P
AD  - Department of Medicine, University of Wisconsin-Madison, School of Medicine and 
      Public Health, Madison, WI 53792-5156, USA.
FAU - Hematti, Peiman
AU  - Hematti P
LA  - eng
GR  - P30 CA014520/CA/NCI NIH HHS/United States
GR  - P30 CA014520-33/CA/NCI NIH HHS/United States
GR  - P40 RR017447/RR/NCRR NIH HHS/United States
GR  - P40 RR 017447/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080107
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - 0 (HLA Antigens)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Cell Differentiation/drug effects/immunology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Embryonic Stem Cells/*cytology/*immunology
MH  - Flow Cytometry
MH  - HLA Antigens/biosynthesis/drug effects
MH  - Humans
MH  - Interferon-gamma/pharmacology
MH  - Leukocytes, Mononuclear/cytology
MH  - Mesenchymal Stem Cells/*cytology/*immunology
MH  - Stromal Cells/*cytology/*immunology
MH  - T-Lymphocytes/immunology
PMC - PMC2315792
MID - NIHMS41498
EDAT- 2008/01/09 09:00
MHDA- 2008/09/06 09:00
PMCR- 2009/03/01
CRDT- 2008/01/09 09:00
PHST- 2007/03/30 00:00 [received]
PHST- 2007/10/05 00:00 [revised]
PHST- 2007/10/29 00:00 [accepted]
PHST- 2008/01/09 09:00 [pubmed]
PHST- 2008/09/06 09:00 [medline]
PHST- 2008/01/09 09:00 [entrez]
PHST- 2009/03/01 00:00 [pmc-release]
AID - S0301-472X(07)00642-X [pii]
AID - 10.1016/j.exphem.2007.10.007 [doi]
PST - ppublish
SO  - Exp Hematol. 2008 Mar;36(3):350-9. doi: 10.1016/j.exphem.2007.10.007. Epub 2008 
      Jan 7.