PMID- 18180053
OWN - NLM
STAT- MEDLINE
DCOM- 20090827
LR  - 20131121
IS  - 1874-1754 (Electronic)
IS  - 0167-5273 (Linking)
VI  - 131
IP  - 1
DP  - 2008 Dec 17
TI  - Effect of folate on neointima formation and matrix metalloproteinase-9 expression 
      after balloon injury in hyperhomocysteinemic rabbits.
PG  - 59-65
LID - 10.1016/j.ijcard.2007.08.138 [doi]
AB  - BACKGROUND: It is known that hyperhomocysteinemia (Hhcy) is associated with the 
      risk of restenosis after angioplasty. Folate can lower plasma homocysteine (Hcy) 
      level and alleviate the Hhcy-induced neointima formation after balloon injury. 
      This study aims to explore the mechanisms of folate in inhibiting neointima 
      formation. METHODS: 24 New Zealand White rabbits were randomly divided into three 
      groups: Control, 2% methionine (Meth) and 2% methionine+folate (Meth+folate). At 
      the end of 8 experimental weeks, all rabbits underwent the balloon injury in 
      abdominal aorta. 4 weeks following this procedure, plasma Hcy concentration, 
      aortic maximal neointimal thickness (NT), neointimal area (NA), medial area (MA), 
      and ratio of neointimal area to medial area (NA/MA), ultrastructure of vascular 
      smooth muscle cells (VSMCs), and matrix metalloproteinase-9 (MMP-9) expression 
      were detected by high performance liquid chromatography, histomorphometric 
      analysis, transmission electron microscope (TEM) and real-time PCR, respectively. 
      RESULTS: It was observed in our study that Hcy concentration, NT, NA, NA/MA and 
      the expression of MMP-9 mRNA were higher in the Meth group than in the control. 
      VSMCs in media exhibited typical synthetic phenotype in the Meth group compared 
      with the transitional state between contractile phenotype and synthetic phenotype 
      in the control group. However, in the rabbits treated with folate, Hcy 
      concentration, NT, NA and MMP-9 mRNA expression were lower than those in the Meth 
      group. The phenotype of VSMCs was close to that in the control group. CONCLUSION: 
      This study suggested that folate could decrease the level of Hcy, reverse the 
      Hhcy-induced exacerbation of neointima formation in rabbits following balloon 
      injury, and the mechanisms in it may be related to the suppressive effect of 
      folate on the expression of MMP-9 mRNA in arterial wall.
FAU - Liu, Fenye
AU  - Liu F
AD  - Department of Traditional Chinese Medicine, Qilu Hospital Affiliated to Shandong 
      University, Jinan 250012, Shandong, PR China.
FAU - Zhang, Jidong
AU  - Zhang J
FAU - Yu, Shudong
AU  - Yu S
FAU - Wang, Rong
AU  - Wang R
FAU - Wang, Bo
AU  - Wang B
FAU - Lai, Liping
AU  - Lai L
FAU - Yin, Huiqiu
AU  - Yin H
FAU - Liu, Guilin
AU  - Liu G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080103
PL  - Netherlands
TA  - Int J Cardiol
JT  - International journal of cardiology
JID - 8200291
RN  - 935E97BOY8 (Folic Acid)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Animals
MH  - Aorta, Abdominal/drug effects/enzymology/injuries/pathology
MH  - Catheterization/*adverse effects
MH  - Folic Acid/*pharmacology/therapeutic use
MH  - Gene Expression Regulation, Enzymologic/drug effects/*physiology
MH  - Hyperhomocysteinemia/drug therapy/*enzymology/pathology
MH  - Male
MH  - Matrix Metalloproteinase 9/*biosynthesis
MH  - Rabbits
MH  - Tunica Intima/drug effects/*enzymology/pathology
EDAT- 2008/01/09 09:00
MHDA- 2009/08/28 09:00
CRDT- 2008/01/09 09:00
PHST- 2007/04/29 00:00 [received]
PHST- 2007/08/09 00:00 [revised]
PHST- 2007/08/18 00:00 [accepted]
PHST- 2008/01/09 09:00 [pubmed]
PHST- 2009/08/28 09:00 [medline]
PHST- 2008/01/09 09:00 [entrez]
AID - S0167-5273(07)01932-8 [pii]
AID - 10.1016/j.ijcard.2007.08.138 [doi]
PST - ppublish
SO  - Int J Cardiol. 2008 Dec 17;131(1):59-65. doi: 10.1016/j.ijcard.2007.08.138. Epub 
      2008 Jan 3.