PMID- 18181015 OWN - NLM STAT- MEDLINE DCOM- 20080501 LR - 20220410 IS - 0920-8569 (Print) IS - 0920-8569 (Linking) VI - 36 IP - 1 DP - 2008 Feb TI - Identification of new genetic subtypes of bovine viral diarrhea virus genotype 1 isolated in Japan. PG - 135-9 LID - 10.1007/s11262-007-0190-0 [doi] AB - A part of the nucleotide sequence of the 5' untranslated region (5'UTR) and E rns region, and the genomic regions encoding for N pro, Core, and E2 of So-like isolates and IS25CP/01 strain which belong to bovine viral diarrhea virus genotype 1 (BVDV-1) were determined and genetic comparisons were made with sequences of other BVDV subgenotypes. Phylogenetic analysis using the 5'UTR and N pro revealed that So-like isolates and IS25CP/01 branched into independent phylogenetic branch. So-like isolates were clustered with Korean BVDV strains taken from DDBL/EMBL/GenBank in the 5'UTR. An additional two amino acid residues were found at the C terminal of the Core region of IS25CP/01. The similarity of amino acid sequence of E2 of So-like isolates and IS25CP/01 to the BVDV-1 reference strain NADL were 78.0-78.5 and 79.0, respectively. Cross-neutralization tests showed significant antigenic differences between So-like isolates and the others (Antigenic similarity (R) value: 2.2-8.8), and IS25CP/01 and the others (R value: 1.6-8.8). So-like viruses and IS25CP/01 differed from the thirteenth subgenotypes (1a-1m) reported by Jackova et al. (2007) and were classified as new genetic subtypes, BVDV-1n (So-like) and 1o (IS25CP/01). FAU - Nagai, Makoto AU - Nagai M AD - Ishikawa Nanbu Livestock Hygiene Service Center, Kanazawa, Ishikawa 920-3101, Japan. FAU - Hayashi, Michiko AU - Hayashi M FAU - Itou, Mika AU - Itou M FAU - Fukutomi, Toyoko AU - Fukutomi T FAU - Akashi, Hiroomi AU - Akashi H FAU - Kida, Hiroshi AU - Kida H FAU - Sakoda, Yoshihiro AU - Sakoda Y LA - eng SI - GENBANK/AB359923 SI - GENBANK/AB359924 SI - GENBANK/AB359925 SI - GENBANK/AB359926 SI - GENBANK/AB359927 SI - GENBANK/AB359928 SI - GENBANK/AB359929 SI - GENBANK/AB359930 SI - GENBANK/AB359931 SI - GENBANK/AB359932 SI - GENBANK/AB359933 SI - GENBANK/AB359934 SI - GENBANK/AB359935 SI - GENBANK/AB359936 SI - GENBANK/AB359937 SI - GENBANK/AB359938 SI - GENBANK/AB359939 SI - GENBANK/AB359940 SI - GENBANK/AB359941 SI - GENBANK/AB359942 SI - GENBANK/AB359943 SI - GENBANK/AB359944 PT - Journal Article DEP - 20080108 PL - United States TA - Virus Genes JT - Virus genes JID - 8803967 SB - IM MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - Bovine Virus Diarrhea-Mucosal Disease/*epidemiology/virology MH - Cattle MH - Diarrhea Virus 1, Bovine Viral/classification/*genetics/immunology/isolation & purification MH - Genetic Variation MH - Genotype MH - Japan/epidemiology MH - Molecular Sequence Data MH - Neutralization Tests MH - Phylogeny MH - Sequence Alignment EDAT- 2008/01/09 09:00 MHDA- 2008/05/02 09:00 CRDT- 2008/01/09 09:00 PHST- 2007/09/30 00:00 [received] PHST- 2007/12/13 00:00 [accepted] PHST- 2008/01/09 09:00 [pubmed] PHST- 2008/05/02 09:00 [medline] PHST- 2008/01/09 09:00 [entrez] AID - 10.1007/s11262-007-0190-0 [doi] PST - ppublish SO - Virus Genes. 2008 Feb;36(1):135-9. doi: 10.1007/s11262-007-0190-0. Epub 2008 Jan 8.