PMID- 18182136
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151105
LR  - 20080109
IS  - 1022-386X (Print)
IS  - 1022-386X (Linking)
VI  - 17
IP  - 12
DP  - 2007 Dec
TI  - Immunohistochemical her-2/ Neu expression with gene amplification by fluorescence 
      in situ hybridization for assessment in breast carcinomas.
PG  - 726-30
AB  - OBJECTIVE: To compare gene amplification of HER-2/neu gene by fluorescence in 
      situ hybridization (FISH) in moderate to strong immunohistochemically (IHS) 
      positive HER-2/neu cases of invasive breast carcinomas. STUDY DESIGN: Cross- 
      sectional study. PLACE AND DURATION OF STUDY: Section of Histopathology, The Aga 
      Khan University Hospital, Karachi, from January 2004 to December 2006. PATIENTS 
      AND METHODS: Forty one (41) diagnosed cases of invasive breast carcinomas were 
      included in this study in which already determined immunohistochemical HER-2/neu 
      expression was scored as either 2+ or 3+, based on the intensity of membranous 
      staining. These cases were further evaluated for gene amplification by FISH. For 
      gene amplification, a ratio of HER-2/CEP Z 2 was accepted as positive gene 
      amplification. RESULTS: Out of a total 41 cases, which were scored as 2+ and 3+ 
      by IHC, 14 cases (34.1%; 95% confidence interval: 19%-49.3% ) showed gene 
      amplification by FISH. Proportion of FISH positivity in IHC 2+ cases alone was 
      found to be 25% (95% confidence interval: 10.5%-41%). In contrast, a majority of 
      IHC 3+ cases (5 of 6) were positive by FISH studies. CONCLUSION: IHC is 
      appropriate for initial HER-2/neu assessment and patients with tumors scored as 
      3+ may be treated alone based on this information provided strict quality control 
      and 95% concordance with FISH assays; however, patients with tumors interpreted 
      as 2+, would benefit from gene amplification by FISH studies for more accurate 
      assessment to avoid inaccurate prognostication and treatment.
FAU - Moatter, Tariq
AU  - Moatter T
AD  - Department of Pathology and Microbiology, The Aga Khan University Hospital, 
      Karachi.
FAU - Din, Zeeshan-Ud-
AU  - Din ZU
FAU - Khilji, Zahida Latif
AU  - Khilji ZL
FAU - Murad, Farzana
AU  - Murad F
FAU - Kayani, Naila
AU  - Kayani N
FAU - Pervez, Shahid
AU  - Pervez S
LA  - eng
PT  - Journal Article
PL  - Pakistan
TA  - J Coll Physicians Surg Pak
JT  - Journal of the College of Physicians and Surgeons--Pakistan : JCPSP
JID - 9606447
EDAT- 2008/01/10 09:00
MHDA- 2008/01/10 09:01
CRDT- 2008/01/10 09:00
PHST- 2007/04/12 00:00 [received]
PHST- 2007/11/27 00:00 [accepted]
PHST- 2008/01/10 09:00 [pubmed]
PHST- 2008/01/10 09:01 [medline]
PHST- 2008/01/10 09:00 [entrez]
AID - 040579197 [pii]
PST - ppublish
SO  - J Coll Physicians Surg Pak. 2007 Dec;17(12):726-30.