PMID- 18182397
OWN - NLM
STAT- MEDLINE
DCOM- 20080317
LR  - 20080229
IS  - 1460-2350 (Electronic)
IS  - 0268-1161 (Linking)
VI  - 23
IP  - 3
DP  - 2008 Mar
TI  - Sperm fluorescence in situ hybridization study of meiotic segregation and an 
      interchromosomal effect in carriers of t(11;18).
PG  - 581-8
LID - 10.1093/humrep/dem345 [doi]
AB  - BACKGROUND: Alanced translocations are associated with infertility, spontaneous 
      abortions and birth defects. METHODS: We report the analysis, by multicolour 
      fluorescence in situ hybridization (FISH), of meiotic segregation and aneuploidy 
      of chromosomes X, Y, 7, 8 and 21 in sperm from three men who are carriers of two 
      different translocations involving chromosomes 11 and 18. A control group 
      comprised ten young, healthy normospermic men. RESULTS: The higher prevalence of 
      alternate segregation followed by adjacent 1, adjacent 2 and 3:1, and other 
      segregants was observed in all three patients. Two carriers of the same 
      translocation differed only in the frequency of adjacent 2 segregation (P < 
      0.01). The carrier of the other translocation showed significantly higher 
      frequency of alternate (P < 0.01) and less adjacent 1 and 3:1 segregation 
      products (P < 0.01). An increased frequency of XY (P < 0.01), YY (P < 0.05) and 
      diploid (P < 0.01) sperm was also detected in the group of translocation carriers 
      compared with the control group. This difference was caused by elevated 
      frequencies of disomy and diploidy in two of our carriers. CONCLUSIONS: The 
      incidence of chromosomally unbalanced or aneuploid gametes varies in the 
      individual translocation carriers even if the same chromosomes are included in 
      the translocation. FISH analysis provides information useful for genetic 
      counseling and assisted reproduction.
FAU - Vozdova, Miluse
AU  - Vozdova M
AD  - Department of Genetics and Reproduction, Veterinary Research Institute, Hudcova 
      70, 621 00 Brno, Czech Republic.
FAU - Oracova, Eva
AU  - Oracova E
FAU - Horinova, Vera
AU  - Horinova V
FAU - Rubes, Jiri
AU  - Rubes J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080107
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
SB  - IM
MH  - Adult
MH  - Aneuploidy
MH  - *Chromosome Segregation
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Chromosomes, Human, Pair 18/*genetics
MH  - Chromosomes, Human, Pair 21/genetics
MH  - Chromosomes, Human, Pair 7/genetics
MH  - Chromosomes, Human, Pair 8/genetics
MH  - Chromosomes, Human, X/genetics
MH  - Chromosomes, Human, Y/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Meiosis/*physiology
MH  - Spermatozoa/*cytology
MH  - Translocation, Genetic/*genetics
EDAT- 2008/01/10 09:00
MHDA- 2008/03/18 09:00
CRDT- 2008/01/10 09:00
PHST- 2008/01/10 09:00 [pubmed]
PHST- 2008/03/18 09:00 [medline]
PHST- 2008/01/10 09:00 [entrez]
AID - dem345 [pii]
AID - 10.1093/humrep/dem345 [doi]
PST - ppublish
SO  - Hum Reprod. 2008 Mar;23(3):581-8. doi: 10.1093/humrep/dem345. Epub 2008 Jan 7.