PMID- 18182683
OWN - NLM
STAT- MEDLINE
DCOM- 20080612
LR  - 20210217
IS  - 0022-2275 (Print)
IS  - 0022-2275 (Linking)
VI  - 49
IP  - 4
DP  - 2008 Apr
TI  - Subcellular localization and dynamics of a digalactolipid-like epitope in 
      Toxoplasma gondii.
PG  - 746-62
LID - 10.1194/jlr.M700476-JLR200 [doi]
AB  - Toxoplasma gondii is a unicellular parasite characterized by unique extracellular 
      and intracellular membrane compartments. The lipid composition of subcellular 
      membranes has not been determined, limiting our understanding of lipid 
      homeostasis, control, and trafficking, a series of processes involved in 
      pathogenesis. In addition to a mitochondrion, Toxoplasma contains a plastid 
      called the apicoplast. The occurrence of a plastid raised the question of the 
      presence of chloroplast galactolipids. Using three independent rabbit and rat 
      antibodies against digalactosyldiacylglycerol (DGDG) from plant chloroplasts, we 
      detected a class of Toxoplasma lipids harboring a digalactolipid-like epitope 
      (DGLE). Immunolabeling characterization supports the notion that the DGLE polar 
      head is similar to that of DGDG. Mass spectrometry analyses indicated that 
      dihexosyl lipids having various hydrophobic moieties (ceramide, diacylglycerol, 
      and acylalkylglycerol) might react with anti-DGDG, but we cannot exclude the 
      possibility that more complex dihexosyl-terminated lipids might also be 
      immunolabeled. DGLE localization was analyzed by immunofluorescence and 
      immunoelectron microscopy and confirmed by subcellular fractionation. No 
      immunolabeling of the apicoplast could be observed. DGLE was scattered in 
      pellicle membrane domains in extracellular tachyzoites and was relocalized to the 
      anterior tip of the cell upon invasion in an actin-dependent manner, providing 
      insights on a possible role in pathogenetic processes. DGLE was detected in other 
      Apicomplexa (i.e., Neospora, Plasmodium, Babesia, and Cryptosporidium).
FAU - Botte, Cyrille
AU  - Botte C
AD  - Unite Mixte de Recherche 5168, Centre National de la Recherche 
      Scientifique-Commissariat a l'Energie, Institut de Recherches en Technologies et 
      Sciences pour le Vivant, 38058 Grenoble, France.
FAU - Saidani, Nadia
AU  - Saidani N
FAU - Mondragon, Ricardo
AU  - Mondragon R
FAU - Mondragon, Monica
AU  - Mondragon M
FAU - Isaac, Giorgis
AU  - Isaac G
FAU - Mui, Ernest
AU  - Mui E
FAU - McLeod, Rima
AU  - McLeod R
FAU - Dubremetz, Jean-Francois
AU  - Dubremetz JF
FAU - Vial, Henri
AU  - Vial H
FAU - Welti, Ruth
AU  - Welti R
FAU - Cesbron-Delauw, Marie-France
AU  - Cesbron-Delauw MF
FAU - Mercier, Corinne
AU  - Mercier C
FAU - Marechal, Eric
AU  - Marechal E
LA  - eng
GR  - 27530 01-20/PHS HHS/United States
GR  - 4328X 01-11/PHS HHS/United States
GR  - P20 RR-16475/RR/NCRR NIH HHS/United States
GR  - R01 TMP 16945 01-20/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20080108
PL  - United States
TA  - J Lipid Res
JT  - Journal of lipid research
JID - 0376606
RN  - 0 (Actins)
RN  - 0 (Antibodies)
RN  - 0 (Epitopes)
RN  - 0 (Galactolipids)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Antibodies/immunology
MH  - Cell Line
MH  - Cell Membrane/metabolism
MH  - Chloroplasts/immunology/metabolism
MH  - Epitopes/*immunology
MH  - Galactolipids/analysis/*immunology/*metabolism
MH  - Intracellular Space/*immunology/*metabolism
MH  - Mass Spectrometry
MH  - Microscopy, Immunoelectron
MH  - Rabbits
MH  - Rats
MH  - Spinacia oleracea/immunology/metabolism
MH  - Toxoplasma/*immunology/*metabolism
EDAT- 2008/01/10 09:00
MHDA- 2008/06/13 09:00
CRDT- 2008/01/10 09:00
PHST- 2008/01/10 09:00 [pubmed]
PHST- 2008/06/13 09:00 [medline]
PHST- 2008/01/10 09:00 [entrez]
AID - S0022-2275(20)42388-0 [pii]
AID - 10.1194/jlr.M700476-JLR200 [doi]
PST - ppublish
SO  - J Lipid Res. 2008 Apr;49(4):746-62. doi: 10.1194/jlr.M700476-JLR200. Epub 2008 
      Jan 8.