PMID- 18186812
OWN - NLM
STAT- MEDLINE
DCOM- 20080129
LR  - 20151119
IS  - 1398-9995 (Electronic)
IS  - 0105-4538 (Linking)
VI  - 63
IP  - 2
DP  - 2008 Feb
TI  - Novel sequences and epitopes of diagnostic value derived from the Anisakis 
      simplex Ani s 7 major allergen.
PG  - 219-25
LID - 10.1111/j.1398-9995.2007.01564.x [doi]
AB  - BACKGROUND: Anisakis simplex allergens may cause severe allergic reactions in 
      infected patients. Human anisakiasis can be specifically diagnosed by detection 
      of immunoglobulin E (IgE) antibodies against O-deglycosylated nAni s 7 allergen 
      captured by monoclonal antibody (mAb) UA3 (UA3-ELISA), although the nature of 
      this important allergen is unknown. The aim of this study was to clone and 
      characterize the Ani s 7 major allergen, and to obtain a recombinant fragment 
      suitable for serodiagnosis. METHODS: An Anisakis cDNA library was screened with 
      mAb UA3 and a cDNA clone (rAni s 7) encoding a 1096-amino acid fragment of Ani s 
      7 (GenBank: EF158010) was identified. Bioinformatic tools and immunological and 
      biochemical techniques were used to characterize the allergen obtained. RESULTS: 
      The rAni s 7 fragment comprised 19 repeats of a novel CX(17-25)CX(9-22)CX(8)CX(6) 
      tandem repeat motif not seen in any previously reported protein sequence. An 
      internal (435)Met-(713)Arg fragment of the rAni s 7 (t-Ani s 7) was expressed in 
      Escherichia coli and evaluated for serodiagnostic utility. Indirect enzyme-linked 
      immunosorbent assay (ELISA) with t-Ani s 7 identified as positive the same 60 
      sera as UA3-ELISA. The sequence MCQCVQKYGTEFCKKRLA from rAni s 7 was identified 
      as the epitope recognized by mAb UA3, and is the target for over 60% of human IgE 
      antibodies that react with O-deglycosylated nAni s 7. CONCLUSIONS: In addition to 
      their clear value for serodiagnosis of human anisakiasis, the nature of the novel 
      sequences and epitopes identified in the Ani s 7 allergen are of interest for a 
      better understanding of the mechanisms operating in Anisakis-induced allergy.
FAU - Rodriguez, E
AU  - Rodriguez E
AD  - Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Madrid, Spain.
FAU - Anadon, A M
AU  - Anadon AM
FAU - Garcia-Bodas, E
AU  - Garcia-Bodas E
FAU - Romaris, F
AU  - Romaris F
FAU - Iglesias, R
AU  - Iglesias R
FAU - Garate, T
AU  - Garate T
FAU - Ubeira, F M
AU  - Ubeira FM
LA  - eng
SI  - GENBANK/EF158010
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Allergens)
RN  - 0 (Antibodies, Helminth)
RN  - 0 (Antigens, Helminth)
RN  - 0 (Epitopes)
RN  - 0 (Helminth Proteins)
SB  - IM
MH  - *Allergens/chemistry/genetics
MH  - *Amino Acid Sequence
MH  - Animals
MH  - Anisakiasis/*diagnosis/immunology
MH  - Anisakis/*immunology
MH  - Antibodies, Helminth/blood
MH  - *Antigens, Helminth/chemistry/genetics/immunology
MH  - Cloning, Molecular
MH  - Epitope Mapping
MH  - Epitopes/*chemistry/immunology
MH  - Gene Library
MH  - Helminth Proteins/chemistry/genetics
MH  - Humans
MH  - Molecular Sequence Data
EDAT- 2008/01/12 09:00
MHDA- 2008/01/30 09:00
CRDT- 2008/01/12 09:00
PHST- 2008/01/12 09:00 [pubmed]
PHST- 2008/01/30 09:00 [medline]
PHST- 2008/01/12 09:00 [entrez]
AID - ALL1564 [pii]
AID - 10.1111/j.1398-9995.2007.01564.x [doi]
PST - ppublish
SO  - Allergy. 2008 Feb;63(2):219-25. doi: 10.1111/j.1398-9995.2007.01564.x.