PMID- 18190236
OWN - NLM
STAT- MEDLINE
DCOM- 20080729
LR  - 20191210
IS  - 0028-2685 (Print)
IS  - 0028-2685 (Linking)
VI  - 55
IP  - 1
DP  - 2008
TI  - Efficacy of high-resolution comparative genomic hybridization (HR-CGH) in 
      detection of chromosomal abnormalities in children with acute leukaemia.
PG  - 23-30
AB  - The efficient detection of chromosomal aberrations in childhood acute leukaemias 
      presents a significant component in the diagnostics of this frequent malignant 
      disease. We used comparative genomic hybridization (CGH) and high-resolution 
      comparative genomic hybridization (HR-CGH) to determine the frequency of 
      chromosomal changes in 33 children with acute leukaemia (AL). The yields of 
      chromosomal abnormalities were compared with the results obtained using 
      conventional cytogenetics (G-banding) and fluorescence in situ hybridization 
      (FISH). Conventional cytogenetics revealed chromosomal changes in 17 (52 %) of 
      studied patients. The employment of FISH together with G-banding analysis 
      identified chromosomal changes in 27 (82 %) of the AL patients investigated. CGH 
      detected changes in DNA copy numbers in 24 (73 %) patients, 40 losses and 67 
      gains were found in total. HR-CGH disclosed 98 losses and 97 gains in 26 (79 %) 
      patients. In comparison with CGH, HR-CGH analyses unveiled 88 new chromosomal 
      aberrations: 58 losses and 30 gains. The most commonly gained chromosomes were 21 
      (22.5 %), X (15 %), 18 (12,5 %) and 17 (10 %). The most common losses involved 
      sub-regions or arms of chromosomes 7 (15 %), 9 (12.5 %), 16, 19 and 1 (10 % 
      each). Cytogenetic and molecular cytogenetic analyses of 33 childhood acute 
      leukaemias revealed chromosomal changes in total 31 (94 %) patients. The 
      evaluation of HR-CGH sensitivity proved that the minimal cell population of 
      malignant cells in which a certain chromosomal change could be found was close to 
      the 20 - 30 % level. Our results confirm the benefits of HR-CGH in detecting 
      chromosomal changes in childhood AL. Supplementing G-banding and FISH with the 
      HR-CGH diagnostic method increases the detection of unbalanced structural 
      chromosomal rearrangements and can reveal small cell clones with gains and losses 
      of whole chromosomes in hyperdiploid AL.
FAU - Vranova, V
AU  - Vranova V
AD  - Department of Genetics and Molecular Biology, Institute of Experimental Biology, 
      Faculty of Science, Masaryk University, Brno, Czech Republic.
FAU - Mentzlova, D
AU  - Mentzlova D
FAU - Oltova, A
AU  - Oltova A
FAU - Linkova, V
AU  - Linkova V
FAU - Zezulkova, D
AU  - Zezulkova D
FAU - Filkova, H
AU  - Filkova H
FAU - Mendelova, D
AU  - Mendelova D
FAU - Sterba, J
AU  - Sterba J
FAU - Kuglik, P
AU  - Kuglik P
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Slovakia
TA  - Neoplasma
JT  - Neoplasma
JID - 0377266
SB  - IM
MH  - Acute Disease
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Leukemia/*genetics
MH  - Male
MH  - Nucleic Acid Hybridization/*methods
EDAT- 2008/01/15 09:00
MHDA- 2008/07/30 09:00
CRDT- 2008/01/15 09:00
PHST- 2008/01/15 09:00 [pubmed]
PHST- 2008/07/30 09:00 [medline]
PHST- 2008/01/15 09:00 [entrez]
PST - ppublish
SO  - Neoplasma. 2008;55(1):23-30.