PMID- 18190513
OWN - NLM
STAT- MEDLINE
DCOM- 20080408
LR  - 20080201
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 10
IP  - 3
DP  - 2008 Mar
TI  - Top-down and bottom-up induced shifts in bacterial abundance, production and 
      community composition in an experimentally divided humic lake.
PG  - 635-52
LID - 10.1111/j.1462-2920.2007.01487.x [doi]
AB  - We examined in situ abundance and activities of the major bacterial groups in the 
      two most distinct compartments of experimentally divided Lake Grosse Fuchskuhle 
      (Germany). The selected south-west (SW) and north-east basin (NE) differ 
      substantially in their major chemical and biological parameters that potentially 
      influence the dynamics and composition of microbial communities. Water from the 
      basins were incubated in dialysis bags, which allowed for a relatively free 
      exchange of nutrients, limiting solutes and low molecular organic matter but 
      fully prevented exchange of organisms. To investigate the effect of top-down and 
      bottom-up manipulations three size fractions of water samples were produced: (i) 
      unfiltered, (ii) pre-filtered through 5.0 microm pore size membranes to remove 
      large particles, as well as grazers and (iii) pre-filtered through 0.8 microm 
      filters to remove all potential bacterivores. One set of dialysis bags was either 
      incubated in acidic SW (rich in humic matter) or in almost neutral NE basin 
      whereas a second set was transferred from the SW to the NE basin and vice versa. 
      Our study revealed pronounced differences in growth rates among the major 
      bacterial groups in relation to the treatments. Members of the 
      Betaproteobacteria, in particular of the subgroup targeted by the BETA2-870 
      probe, were highly abundant in both basins, and most of them belonged to the 
      Polynucleobacter necessesarius subcluster PnecC. Their specific growth rates 
      surprisingly increased in all treatments when being transplanted into the acidic 
      SW basin, indicating that pH and humic substances greatly affected growth of this 
      particular group in the lake. In contrast, members of the 
      Sphingobacteria/Flavobacteria group of the Bacteroidetes (both basins) as well as 
      Actinobacteria (SW basin) were less abundant, especially in the presence of 
      flagellates (< 5.0 microm treatments). However, because of their extremely low 
      initial numbers, grazing of heterotrophic nanoflagellates mostly controlled only 
      a small part of the bacterial production (< or = 12%).
FAU - Grossart, Hans-Peter
AU  - Grossart HP
AD  - Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Alte Fischerhuette 
      2, D-16775 Stechlin, Germany. hgrossart@igb-berlin.de
FAU - Jezbera, Jan
AU  - Jezbera J
FAU - Hornak, Karel
AU  - Hornak K
FAU - Hutalle, Kristine Michelle L
AU  - Hutalle KM
FAU - Buck, Ulrike
AU  - Buck U
FAU - Simek, Karel
AU  - Simek K
LA  - eng
SI  - GENBANK/EF394793
SI  - GENBANK/EF394794
SI  - GENBANK/EF394795
SI  - GENBANK/EF394796
SI  - GENBANK/EF394797
SI  - GENBANK/EF394798
SI  - GENBANK/EF394799
SI  - GENBANK/EF394800
SI  - GENBANK/EF394801
SI  - GENBANK/EF394802
SI  - GENBANK/EF394803
SI  - GENBANK/EF394804
SI  - GENBANK/EF394805
SI  - GENBANK/EF394806
SI  - GENBANK/EF394807
SI  - GENBANK/EF394808
SI  - GENBANK/EF394809
SI  - GENBANK/EF394810
SI  - GENBANK/EF394811
SI  - GENBANK/EF394812
SI  - GENBANK/EF394813
SI  - GENBANK/EF394814
SI  - GENBANK/EF394815
SI  - GENBANK/EF394816
SI  - GENBANK/EF394817
SI  - GENBANK/EF394818
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080107
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Humic Substances)
SB  - IM
MH  - Bacteria/*chemistry/*genetics/growth & development
MH  - *Ecosystem
MH  - Filtration
MH  - Fresh Water/chemistry/*microbiology
MH  - Humic Substances
MH  - Molecular Sequence Data
MH  - Plankton/isolation & purification
EDAT- 2008/01/15 09:00
MHDA- 2008/04/09 09:00
CRDT- 2008/01/15 09:00
PHST- 2008/01/15 09:00 [pubmed]
PHST- 2008/04/09 09:00 [medline]
PHST- 2008/01/15 09:00 [entrez]
AID - EMI1487 [pii]
AID - 10.1111/j.1462-2920.2007.01487.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2008 Mar;10(3):635-52. doi: 10.1111/j.1462-2920.2007.01487.x. 
      Epub 2008 Jan 7.