PMID- 18190896
OWN - NLM
STAT- MEDLINE
DCOM- 20080603
LR  - 20191210
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 1193
DP  - 2008 Feb 8
TI  - Protective effect of nitric oxide synthase inhibition or antioxidants on brain 
      oxidative damage caused by intracerebroventricular arginine administration.
PG  - 120-7
LID - 10.1016/j.brainres.2007.11.052 [doi]
AB  - We have previously demonstrated that acute arginine administration induces 
      oxidative stress and compromises energy metabolism in rat hippocampus. In the 
      present study, we initially investigated the effect of intracerebroventricular 
      infusion of arginine (0.1, 0.5 and 1.5 mM solution) on Na(+),K(+)-ATPase activity 
      and on some parameters of oxidative stress, namely thiobarbituric acid-reactive 
      substances (TBA-RS) and total radical-trapping antioxidant parameter (TRAP) in 
      the hippocampus of rats. Results showed that 1.5 mM arginine solution 
      significantly increases TBA-RS and reduces Na(+),K(+)-ATPase activity and TRAP in 
      the rat hippocampus. We also evaluated the influence of the nitric oxide synthase 
      inhibitor, N(omega)-nitro-L-arginine methyl ester (L-NAME), and antioxidants, 
      namely alpha-tocopherol plus ascorbic acid, on the effects elicited by arginine 
      on Na(+),K(+)-ATPase activity, TBA-RS and TRAP. Results showed that treatment 
      with alpha-tocopherol plus ascorbic acid per se did not alter these parameters 
      but prevented these effects. Furthermore, intracerebroventricular infusion of 
      L-NAME prevented the inhibition caused by arginine on Na(+),K(+)-ATPase activity, 
      as well as the increased of TBA-RS. Our findings indicate that 
      intracerebroventricular infusion of arginine induces oxidative stress in rat 
      hippocampus and that the inhibition of Na(+),K(+)-ATPase activity caused by this 
      amino acid was probably mediated by NO and/or its derivatives ONOO(-) and/or 
      other free radicals. Finally, we suggest that the administration of antioxidants 
      should be considered as an adjuvant therapy to specific diets in 
      hyperargininemia.
FAU - Delwing, Debora
AU  - Delwing D
AD  - Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade 
      Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003, 
      Porto Alegre, RS, Brazil.
FAU - Delwing, Daniela
AU  - Delwing D
FAU - Bavaresco, Caren S
AU  - Bavaresco CS
FAU - Wyse, Angela T S
AU  - Wyse AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071205
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Amidines)
RN  - 0 (Antioxidants)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 7381JDR72F (2,2'-azobis(2-amidinopropane))
RN  - 94ZLA3W45F (Arginine)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
RN  - H4N855PNZ1 (alpha-Tocopherol)
RN  - PQ6CK8PD0R (Ascorbic Acid)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - Amidines/metabolism
MH  - Analysis of Variance
MH  - Animals
MH  - Antioxidants/*therapeutic use
MH  - Arginine/administration & dosage
MH  - Ascorbic Acid/therapeutic use
MH  - Brain Injuries/chemically induced/pathology/*prevention & control
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Drug Interactions
MH  - Enzyme Inhibitors/*therapeutic use
MH  - Hippocampus/drug effects/metabolism
MH  - Injections, Intraventricular/methods
MH  - Male
MH  - NG-Nitroarginine Methyl Ester/*therapeutic use
MH  - Oxidative Stress/*drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - Sodium-Potassium-Exchanging ATPase/metabolism
MH  - Thiobarbituric Acid Reactive Substances/metabolism
MH  - alpha-Tocopherol/therapeutic use
EDAT- 2008/01/15 09:00
MHDA- 2008/06/05 09:00
CRDT- 2008/01/15 09:00
PHST- 2007/10/02 00:00 [received]
PHST- 2007/11/23 00:00 [revised]
PHST- 2007/11/27 00:00 [accepted]
PHST- 2008/01/15 09:00 [pubmed]
PHST- 2008/06/05 09:00 [medline]
PHST- 2008/01/15 09:00 [entrez]
AID - S0006-8993(07)02858-2 [pii]
AID - 10.1016/j.brainres.2007.11.052 [doi]
PST - ppublish
SO  - Brain Res. 2008 Feb 8;1193:120-7. doi: 10.1016/j.brainres.2007.11.052. Epub 2007 
      Dec 5.