PMID- 18193443
OWN - NLM
STAT- MEDLINE
DCOM- 20090106
LR  - 20181113
IS  - 0937-4477 (Print)
IS  - 1434-4726 (Electronic)
IS  - 0937-4477 (Linking)
VI  - 265 Suppl 1
IP  - Suppl 1
DP  - 2008 Jul
TI  - Short-fragment PCR assay for highly sensitive broad-spectrum detection of human 
      papillomaviruses in laryngeal squamous cell carcinoma and normal mucosa: 
      clinico-pathological evaluation.
PG  - S89-96
LID - 10.1007/s00405-007-0569-5 [doi]
AB  - The aim of the study was to determine the prevalence and genotypes of HPV 
      infection in laryngeal cancer specimens, normal mucosa obtained from the surgical 
      margin and laryngeal nodules using a novel high sensitive and specific SPF(10) 
      HPV DNA test, PCR/DEIA method and INNO-LiPA genotyping assay. The correlation 
      between HPV presence and clinico-pathological features was analyzed. Tissue 
      samples were collected from 93 primary laryngeal squamous cell carcinoma (LSCC), 
      49 specimens of normal mucosa and from 22 specimens of laryngeal nodules serving 
      as control group. HPV DNA was amplified by the short PCR fragment (SPF(10)) 
      primer set using HPV DNA enzyme immunoassay (DNA/DEIA) method and INNO-LiPA HPV 
      genotyping assay. Human papillomavirus was detected in 33 (35.5%) of the 93 
      samples from LSCC, in 4 (8.2%) of 49 samples of the normal mucosa and it was not 
      detected in any of the sample from the control group. Twenty-eight of 33 (81.8%) 
      were positive for HPV-16, 6 of 33 (18.2%) were positive for HPV-18 and 5 of 33 
      (15.1%) were positive for HPV-33. Multiple infection was found in 5 of 33 
      (15.1%); 3 samples were positive for HPV-16 and HPV-33, 2 samples for HPV-16 and 
      HPV-18. There was a statistically significant correlation between the presence of 
      HPV in LSCC tumors and in control group samples and between the presence of HPV 
      in the tumors and normal mucosa from the free surgical margin. The presence of 
      HPV infection in 35.5% of the cases suggests a possible role in the etiology of 
      laryngeal cancer and supports the role of high-risk types of HPV (16, 18 and 33) 
      in LSCC. HPV infection is not likely to influence survival rates as an 
      independent prognostic factor in patients with laryngeal cancer.
FAU - Morshed, Kamal
AU  - Morshed K
AD  - Department of Otolaryngology Head and Neck Surgery, Medical University of Lublin, 
      ul. Jaczewskiego 8, 20-954 Lublin, Poland. kamal1@op.pl
FAU - Polz-Dacewicz, Malgorzata
AU  - Polz-Dacewicz M
FAU - Szymanski, Marcin
AU  - Szymanski M
FAU - Polz, Dorota
AU  - Polz D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080105
PL  - Germany
TA  - Eur Arch Otorhinolaryngol
JT  - European archives of oto-rhino-laryngology : official journal of the European 
      Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the 
      German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
JID - 9002937
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Alphapapillomavirus/genetics/*isolation & purification
MH  - Carcinoma, Squamous Cell/pathology/*virology
MH  - DNA, Viral/isolation & purification
MH  - Electrophoresis, Agar Gel
MH  - Human papillomavirus 16/isolation & purification
MH  - Human papillomavirus 18/isolation & purification
MH  - Humans
MH  - Laryngeal Neoplasms/*virology
MH  - Larynx
MH  - Papillomavirus Infections/*complications
MH  - Paraffin Embedding
MH  - Polymerase Chain Reaction/*methods
MH  - Sensitivity and Specificity
PMC - PMC2441493
EDAT- 2008/01/15 09:00
MHDA- 2009/01/07 09:00
PMCR- 2008/06/27
CRDT- 2008/01/15 09:00
PHST- 2007/10/07 00:00 [received]
PHST- 2007/12/20 00:00 [accepted]
PHST- 2008/01/15 09:00 [pubmed]
PHST- 2009/01/07 09:00 [medline]
PHST- 2008/01/15 09:00 [entrez]
PHST- 2008/06/27 00:00 [pmc-release]
AID - 569 [pii]
AID - 10.1007/s00405-007-0569-5 [doi]
PST - ppublish
SO  - Eur Arch Otorhinolaryngol. 2008 Jul;265 Suppl 1(Suppl 1):S89-96. doi: 
      10.1007/s00405-007-0569-5. Epub 2008 Jan 5.