PMID- 18198380
OWN - NLM
STAT- MEDLINE
DCOM- 20080404
LR  - 20200228
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 89
IP  - Pt 2
DP  - 2008 Feb
TI  - The nsp1alpha and nsp1 papain-like autoproteinases are essential for porcine 
      reproductive and respiratory syndrome virus RNA synthesis.
PG  - 494-499
LID - 10.1099/vir.0.83253-0 [doi]
AB  - The two N-terminal cleavage products, nsp1alpha and nsp1beta, of the replicase 
      polyproteins of porcine reproductive and respiratory syndrome virus (PRRSV) each 
      contain a papain-like autoproteinase domain, which have been named PCPalpha and 
      PCPbeta, respectively. To assess their role in the PRRSV life cycle, 
      substitutions and deletions of the presumed catalytic cysteine and histidine 
      residues of PCPalpha and PCPbeta were introduced into a PRRSV infectious cDNA 
      clone. Mutations that inactivated PCPalpha activity completely blocked subgenomic 
      mRNA synthesis, but did not affect genome replication. In contrast, mutants in 
      which PCPbeta activity was blocked proved to be non-viable and no sign of viral 
      RNA synthesis could be detected, indicating that the correct processing of the 
      nsp1beta/nsp2 cleavage site is essential for PRRSV genome replication. In 
      conclusion, the data presented here show that a productive PRRSV life cycle 
      depends on the correct processing of both the nsp1alpha/nsp1beta and 
      nsp1beta/nsp2 junctions.
FAU - Kroese, Michiel V
AU  - Kroese MV
AD  - Wageningen UR, Animal Sciences Group, Division of Infectious Diseases, PO Box 65, 
      8200 AB Lelystad, The Netherlands.
FAU - Zevenhoven-Dobbe, Jessika C
AU  - Zevenhoven-Dobbe JC
AD  - Molecular Virology Laboratory, Department of Medical Microbiology, Center of 
      Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
FAU - Bos-de Ruijter, Judy N A
AU  - Bos-de Ruijter JNA
AD  - Wageningen UR, Animal Sciences Group, Division of Infectious Diseases, PO Box 65, 
      8200 AB Lelystad, The Netherlands.
FAU - Peeters, Ben P H
AU  - Peeters BPH
AD  - Wageningen UR, Animal Sciences Group, Division of Infectious Diseases, PO Box 65, 
      8200 AB Lelystad, The Netherlands.
FAU - Meulenberg, Janneke J M
AU  - Meulenberg JJM
AD  - Wageningen UR, Animal Sciences Group, Division of Infectious Diseases, PO Box 65, 
      8200 AB Lelystad, The Netherlands.
FAU - Cornelissen, Lisette A H M
AU  - Cornelissen LAHM
AD  - Wageningen UR, Animal Sciences Group, Division of Infectious Diseases, PO Box 65, 
      8200 AB Lelystad, The Netherlands.
FAU - Snijder, Eric J
AU  - Snijder EJ
AD  - Molecular Virology Laboratory, Department of Medical Microbiology, Center of 
      Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Polyproteins)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Nonstructural Proteins)
RN  - EC 3.4.- (Peptide Hydrolases)
RN  - EC 3.4.22.2 (Papain)
SB  - IM
MH  - Animals
MH  - Papain/chemistry
MH  - Peptide Hydrolases/*metabolism/physiology
MH  - Polyproteins/genetics
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*metabolism
MH  - RNA, Viral/*biosynthesis/genetics
MH  - Swine
MH  - Viral Nonstructural Proteins/genetics/*metabolism
MH  - Virus Replication/*physiology
EDAT- 2008/01/17 09:00
MHDA- 2008/04/05 09:00
CRDT- 2008/01/17 09:00
PHST- 2008/01/17 09:00 [pubmed]
PHST- 2008/04/05 09:00 [medline]
PHST- 2008/01/17 09:00 [entrez]
AID - 10.1099/vir.0.83253-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2008 Feb;89(Pt 2):494-499. doi: 10.1099/vir.0.83253-0.