PMID- 18198696
OWN - NLM
STAT- MEDLINE
DCOM- 20080303
LR  - 20190923
IS  - 1061-4303 (Print)
IS  - 1061-4303 (Linking)
VI  - 79
IP  - 13
DP  - 2007 Dec
TI  - Nitrifying community analysis in a single submerged attached-growth bioreactor 
      for treatment of high-ammonia waste stream.
PG  - 2510-8
AB  - This study investigated the nitrifying community structure in a single-stage 
      submerged attached-growth bioreactor (SAGB) that successfully achieved stable 
      nitrogen removal over nitrite of a high-strength ammonia wastewater. The reactor 
      was operated with intermittent aeration and external carbon addition (methanol). 
      With influent ammonia and total Kjeldahl nitrogen ranging from 537 to 968 mg/L 
      and 643 to 1510 mg/L, respectively, 85% nitrogen removal was obtained, and 
      effluent was dominated by nitrite (NO2-/NOx > 0.95). Nitrifying community 
      analysis using fluorescence in situ hybridization (FISH), with a hierarchical set 
      of probes targeting known ammonia-oxidizing bacteria (AOB) within 
      beta-proteobacteria, showed that the AOB community of the biofilter consists 
      almost entirely of members of the Nitrosomonas europaea/eutropha and the 
      Nitrosococcus mobilis lineages. Image analysis of FISH pictures was used to 
      quantify the identified AOB, and it was estimated that Nitrosomonas 
      europaea/eutropha-like AOB accounted for 4.3% of the total volume of the biofilm, 
      while Nitrosococcus mobilis-like AOB made up 1.2%; these numbers summed up to a 
      total AOB fraction of 5.5% of the total volume on the biofilm. Nitrite-oxidizing 
      bacteria (NOB) were not detectable in the biofilm samples with probes for either 
      Nitrospira sp. or Nitrobacter sp., which indicated that NOB were either absent 
      from the biofilters or present in numbers below the detection limit of FISH (< 
      0.1% of the total biofilm). Nitrite oxidizers were likely outcompeted from the 
      system because of the free ammonia inhibition and the possibility that the 
      aeration period (from intermittent aeration) was not sufficiently long for the 
      NOB to be released from the competition for oxygen with heterotrophs and AOB. The 
      nitrogen removal via nitrite in a SAGB reactor described in this study is 
      applicable for high-ammonia-strength wastewater treatment, such as centrate or 
      industrial wastes.
FAU - Gu, April Z
AU  - Gu AZ
AD  - Department of Civil and Environmental Engineering, Northeastern University, 
      Boston, Massachusetts, USA. april@coe.neu.edu
FAU - Pedros, Philip B
AU  - Pedros PB
FAU - Kristiansen, Anja
AU  - Kristiansen A
FAU - Onnis-Hayden, Annalisa
AU  - Onnis-Hayden A
FAU - Schramm, Andreas
AU  - Schramm A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Water Environ Res
JT  - Water environment research : a research publication of the Water Environment 
      Federation
JID - 9886167
RN  - 0 (Nitrites)
RN  - 7664-41-7 (Ammonia)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Aerobiosis
MH  - Ammonia/*metabolism
MH  - Betaproteobacteria/metabolism
MH  - Biofilms/growth & development
MH  - Bioreactors/*microbiology
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Nitrites/metabolism
MH  - Nitrobacter/*metabolism
MH  - Nitrogen/metabolism
MH  - Nitrosomonas/*metabolism
MH  - Nitrosomonas europaea/metabolism
MH  - Waste Disposal, Fluid
MH  - Waste Management
EDAT- 2008/01/18 09:00
MHDA- 2008/03/04 09:00
CRDT- 2008/01/18 09:00
PHST- 2008/01/18 09:00 [pubmed]
PHST- 2008/03/04 09:00 [medline]
PHST- 2008/01/18 09:00 [entrez]
AID - 10.2175/106143007x254566 [doi]
PST - ppublish
SO  - Water Environ Res. 2007 Dec;79(13):2510-8. doi: 10.2175/106143007x254566.