PMID- 18201529
OWN - NLM
STAT- MEDLINE
DCOM- 20101104
LR  - 20080118
IS  - 0412-4081 (Print)
IS  - 0412-4081 (Linking)
VI  - 43
IP  - 10
DP  - 2007 Oct
TI  - [Expression of stromal cell-derived factor 1 is increased in the retina of 
      experimental diabetic rats].
PG  - 912-6
AB  - OBJECTIVE: To investigate the expression of stromal cell-derived factor 1 (SDF-1) 
      in retina of experimental diabetic rats. METHODS: Thirty four healthy male Wistar 
      rats of six to eight weeks old were chosen and divided randomly into 3 groups: 
      normal control (CON, n = 8), diabetes mellitus 2 months (DM2M, n = 14) and 3 
      months (DM3M, n = 12). Diabetes was induced by a single intraperitoneal injection 
      of streptozotocin (STZ) (60 mg/kg). At the end point, immunochemistry and digital 
      photomicrography analysis were employed to localize and quantitate the expression 
      of SDF-1 in the retinas. The ratios of integrated optical density (IOD) to area 
      of interest (AOI) were calculated to evaluate the intensities of positive 
      staining. Values of z and t, as well as P value of each, were determined by 
      Wilcoxon-Mann-Whitney test and Student's t-test, respectively. Immunofluorescent 
      double-staining was used to indicate the co-expression of SDF-1 and 
      hypoxia-inducible factor-1 (HIF-1), a pivotal transcription factor activated by 
      hypoxia. The SDF-1 mRNA of the retina from DM2M (n = 4) and CON (n = 2) was 
      detected by real-time RT-PCR. Copies of SDF-1 mRNA were then adjusted by the 
      copies of beta-actin mRNA in each sample. Differences between groups were 
      compared by a Student's t test. RESULTS: Positive immunohistochemical staining 
      for SDF-1 was found in each group, most of which located in the inner part of the 
      retina, especially at the inner nuclear layer (INL) and ganglion cell layer 
      (GCL). The inter-quartile range of IOD/AOI of SDF-1 was between 0.6 x 10(-2) to 
      1.5 x 10(-2) in diabetic group, and 1.1 x 10(-3) to 2.4 x 10(-3) in control group 
      (z = -3.359, P < 0.001). There was no significant difference between DM2M and 
      DM3M (t = 0.952, P > 0.05). Co-expression of SDF-1 and HIF-1 was found in some 
      cells in the inner part of the retina of diabetic rats. Copies of SDF-1 mRNA in 
      DM2M were 2.3 x 10(-4) +/- 1.2 x 10(-4), and those in CON were 2.9 x 10(-4) +/- 
      1.0 x 10(-4). The ratios of them to beta-actin were 3.3 x 10(-2) +/- 8.5 x 10(-3) 
      for DM2M and 1.7 x 10(-2) +/- 5.7 x 10(-3) for CON (t = 2.33, P < 0.05). 
      CONCLUSION: Possibly promoted by HIF-1, expression of SDF-1 is increased in the 
      retinas of diabetic rats, which may play an important role in the mechanism of 
      diabetic retinopathy.
FAU - Yuan, Yuan-Zhi
AU  - Yuan YZ
AD  - Department of Ophthalmology, Zhongshan Hospital, Fudan University, Shanghai, 
      China.
FAU - Yuan, Fei
AU  - Yuan F
FAU - Li, Lei
AU  - Li L
FAU - Wang, Yang
AU  - Wang Y
FAU - Tong, Bei-Yan
AU  - Tong BY
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yan Ke Za Zhi
JT  - [Zhonghua yan ke za zhi] Chinese journal of ophthalmology
JID - 16210540R
RN  - 0 (Chemokine CXCL12)
SB  - IM
MH  - Animals
MH  - Chemokine CXCL12/*metabolism
MH  - Diabetes Mellitus, Experimental/*metabolism
MH  - Diabetic Retinopathy/*metabolism
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Retina/*metabolism
EDAT- 2008/01/19 09:00
MHDA- 2010/11/05 06:00
CRDT- 2008/01/19 09:00
PHST- 2008/01/19 09:00 [pubmed]
PHST- 2010/11/05 06:00 [medline]
PHST- 2008/01/19 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Yan Ke Za Zhi. 2007 Oct;43(10):912-6.