PMID- 18203052
OWN - NLM
STAT- MEDLINE
DCOM- 20080415
LR  - 20181201
IS  - 0032-0943 (Print)
IS  - 0032-0943 (Linking)
VI  - 74
IP  - 1
DP  - 2008 Jan
TI  - Micellar electrokinetic chromatography for the quantitative analysis of 
      flavonoids in the radix of Astragalus membranaceus var. mongholicus.
PG  - 84-9
LID - 10.1055/s-2008-1034291 [doi]
AB  - The separation and determination of eight flavonoids in the radix of Astragalus 
      membranaceus (Fisch.) Bge var. mongholicus (Bge.) Hsiao was achieved by using 
      micellar electrokinetic chromatography (MEKC) with a diode array detector (DAD). 
      The influence of background electrolyte (BGE) concentrations and pH, surfactant 
      concentrations, organic solvents, temperature, and voltage on the separate 
      procedure was systematically investigated. The optimal resolution was obtained 
      with a micellar phase consisting of 100 mM sodium cholate, 25 % (v/v) 
      acetonitrile, 20 mM Na2B4O7, and 20 mM H3BO3 buffer at pH 9.2 by using a fused 
      silica capillary at + 25 kV and 25 degrees C. A high reproducibility and good 
      linearity ( R2 > 0.9978) were obtained over the concentration range tested. The 
      relative standard deviations (R.S.D.s) from intra-day and inter-day precision for 
      injection were less than 4.42 %. Six plant samples from different locations were 
      then analyzed for the flavonoids by this newly developed MEKC method.
FAU - Song, Yue
AU  - Song Y
AD  - Department of Chinese Medicine Science & Engineering, Zhejiang University, 
      Hangzhou, People's Republic of China.
FAU - Li, Ping
AU  - Li P
FAU - Wang, Duo
AU  - Wang D
FAU - Cheng, Yi-Yu
AU  - Cheng YY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080117
PL  - Germany
TA  - Planta Med
JT  - Planta medica
JID - 0066751
RN  - 0 (Flavonoids)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - *Astragalus propinquus
MH  - Chromatography, Micellar Electrokinetic Capillary
MH  - Flavonoids/chemistry
MH  - Humans
MH  - *Phytotherapy
MH  - Plant Extracts/*chemistry
EDAT- 2008/01/19 09:00
MHDA- 2008/04/16 09:00
CRDT- 2008/01/19 09:00
PHST- 2008/01/19 09:00 [pubmed]
PHST- 2008/04/16 09:00 [medline]
PHST- 2008/01/19 09:00 [entrez]
AID - 10.1055/s-2008-1034291 [doi]
PST - ppublish
SO  - Planta Med. 2008 Jan;74(1):84-9. doi: 10.1055/s-2008-1034291. Epub 2008 Jan 17.