PMID- 18205991
OWN - NLM
STAT- MEDLINE
DCOM- 20090119
LR  - 20090520
IS  - 1475-2662 (Electronic)
IS  - 0007-1145 (Linking)
VI  - 100
IP  - 1
DP  - 2008 Jul
TI  - Bacterial population dynamics and faecal short-chain fatty acid (SCFA) 
      concentrations in healthy humans.
PG  - 138-46
LID - 10.1017/S0007114507886351 [doi]
AB  - Fermentation products, SCFA, particularly butyrate, are considered a sign of 
      'good' bowel health but the influence of bacterial population composition and 
      diet on inter-individual difference in metabolites and colonic health is poorly 
      understood. Faecal specimens were collected weekly from eight healthy human 
      volunteers over 12 weeks. Dietary intake was self-reported and ten macronutrient 
      factors were analysed at selected weekly periods. Faecal weight, pH and moisture 
      were recorded, and SCFA concentrations were measured in all samples. From each 
      specimen, DNA was prepared and eubacterial 16S rRNA gene PCR performed. Bacterial 
      population profiles were captured by denaturing gradient gel electrophoresis 
      (DGGE) of PCR products, and multivariate statistical analysis was performed. 
      Faecal weight, pH and moisture varied widely within and between individuals. 
      Average total SCFA concentrations over 12 weeks ranged from 36.9 to 144.4 mmol/kg 
      in 48 h specimens and faecal butyrate concentrations ranged from 1.8 to 48.5 
      mmol/kg. Two individuals with butyrate concentrations below 10 mmol/kg were 
      considered to be 'low butyrate types' and may represent an at-risk population for 
      bowel health. Dietary fat, sugar and carbohydrate showed weak correlation with 
      SCFA (R - 0.612, P = 0.015; R 0.607, P = 0.016; R 0.610, P = 0.016, respectively) 
      and butyrate concentrations (R - 0.593, P = 0.02; R 0.504, P = 0.054; R 0.528, P 
      = 0.043, respectively). Multivariate analysis of DGGE bacterial profiles 
      demonstrated concise and repeated grouping of intra-individual samples, but these 
      were combined with distinct inter-individual differences (analysis of 
      similarities P < 0.001, R > or = 0.99) The exact relationship of these SCFA 
      values to the overall bacterial profiles and SCFA-producer bacterial groups was 
      not direct nor linear.
FAU - McOrist, Alexandra L
AU  - McOrist AL
AD  - Preventative Health Flagship, CSIRO Human Nutrition, PO Box 10041, Adelaide BC, 
      SA 5000, Australia. sandi.mcorist@csiro.au
FAU - Abell, Guy C J
AU  - Abell GC
FAU - Cooke, Caroline
AU  - Cooke C
FAU - Nyland, Kerry
AU  - Nyland K
LA  - eng
PT  - Journal Article
DEP - 20080121
PL  - England
TA  - Br J Nutr
JT  - The British journal of nutrition
JID - 0372547
RN  - 0 (DNA, Bacterial)
RN  - 0 (Fatty Acids, Volatile)
SB  - IM
MH  - Adult
MH  - Bacteria/*growth & development/isolation & purification/metabolism
MH  - DNA, Bacterial/isolation & purification
MH  - Defecation
MH  - Diet
MH  - Electrophoresis, Polyacrylamide Gel/methods
MH  - Fatty Acids, Volatile/*metabolism
MH  - Feces/*chemistry/*microbiology
MH  - Female
MH  - Food Analysis/methods
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction/methods
EDAT- 2008/01/22 09:00
MHDA- 2009/01/20 09:00
CRDT- 2008/01/22 09:00
PHST- 2008/01/22 09:00 [pubmed]
PHST- 2009/01/20 09:00 [medline]
PHST- 2008/01/22 09:00 [entrez]
AID - S0007114507886351 [pii]
AID - 10.1017/S0007114507886351 [doi]
PST - ppublish
SO  - Br J Nutr. 2008 Jul;100(1):138-46. doi: 10.1017/S0007114507886351. Epub 2008 Jan 
      21.