PMID- 18207277
OWN - NLM
STAT- MEDLINE
DCOM- 20080623
LR  - 20191210
IS  - 1872-8227 (Electronic)
IS  - 0168-8227 (Linking)
VI  - 80
IP  - 1
DP  - 2008 Apr
TI  - Determination of serum 7-ketocholesterol concentrations and their relationships 
      with coronary multiple risks in diabetes mellitus.
PG  - 63-8
LID - 10.1016/j.diabres.2007.10.023 [doi]
AB  - Oxysterols have cytotoxic effects and contribute to the development of 
      atherosclerosis. To examine association between 7-ketocholesterol and diabetes 
      mellitus, and other coronary risk factors, we developed a reliable quantitative 
      method to measure serum 7-ketocholesterol (s-7KCHO) and studied s-7KCHO in 
      patients with type 2 diabetes mellitus (T2DM). The s-7KCHO was detected by gas 
      chromatography-mass spectrometry assay. The s-7KCHO was significantly higher in 
      patients with T2DM (n=137, 33.8 ng/ml) compared to non-diabetic healthy subjects 
      (n=89, 16.1 ng/ml). Patients with T2DM were divided into two groups with two or 
      more than two risk factors (defined as multiple risk factors group) and with zero 
      or one risk factor (non-multiple risk factors group). The s-7KCHO was 
      significantly higher in multiple risk factors group (39.5 ng/ml) compared to 
      non-multiple risk factors (30.1 ng/ml). Among patients with multiple risk factors 
      group, s-7KCHO was significantly higher in patients with high low-density 
      lipoprotein cholesterol (LDL-C) levels (45.1+/-5.9 ng/ml) compared to those with 
      normal LDL-C levels (35.3+/-7.0 ng/ml). Furthermore, s-7KCHO increased according 
      to the number of concurrent coronary risk factors. These results suggest that 
      serum 7-ketocholesterol levels may depend on the multiple risk factors and serum 
      LDL-C levels.
FAU - Endo, Kei
AU  - Endo K
AD  - The Center of Diabetes, Endocrinology, and Metabolism, Toho University Medical 
      Center Sakura Hospital, 564-1 Shimoshizu, Sakura-City, Chiba, Japan.
FAU - Oyama, Tomokazu
AU  - Oyama T
FAU - Saiki, Atsuhito
AU  - Saiki A
FAU - Ban, Noriko
AU  - Ban N
FAU - Ohira, Masahiro
AU  - Ohira M
FAU - Koide, Nobukiyo
AU  - Koide N
FAU - Murano, Takeyoshi
AU  - Murano T
FAU - Watanabe, Hitoshi
AU  - Watanabe H
FAU - Nishii, Manabu
AU  - Nishii M
FAU - Miura, Minoru
AU  - Miura M
FAU - Sekine, Kyoichi
AU  - Sekine K
FAU - Miyashita, Yoh
AU  - Miyashita Y
FAU - Shirai, Koji
AU  - Shirai K
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Validation Study
DEP - 20080122
PL  - Ireland
TA  - Diabetes Res Clin Pract
JT  - Diabetes research and clinical practice
JID - 8508335
RN  - 0 (Biomarkers)
RN  - 0 (Cholesterol, LDL)
RN  - 0 (Ketocholesterols)
RN  - O7676FE78M (7-ketocholesterol)
SB  - IM
MH  - Biomarkers/analysis/*blood
MH  - Chemistry, Clinical/instrumentation/standards
MH  - Cholesterol, LDL/blood
MH  - Coronary Artery Disease/*blood/epidemiology
MH  - Diabetes Mellitus, Type 2/*blood/epidemiology
MH  - Diabetic Angiopathies/*blood/epidemiology
MH  - Female
MH  - Gas Chromatography-Mass Spectrometry/methods/*standards
MH  - Humans
MH  - Ketocholesterols/analysis/*blood
MH  - Male
MH  - Metabolic Syndrome/blood/epidemiology
MH  - Middle Aged
MH  - Reproducibility of Results
MH  - Risk Factors
EDAT- 2008/01/22 09:00
MHDA- 2008/06/24 09:00
CRDT- 2008/01/22 09:00
PHST- 2007/09/28 00:00 [received]
PHST- 2007/10/11 00:00 [accepted]
PHST- 2008/01/22 09:00 [pubmed]
PHST- 2008/06/24 09:00 [medline]
PHST- 2008/01/22 09:00 [entrez]
AID - S0168-8227(07)00530-X [pii]
AID - 10.1016/j.diabres.2007.10.023 [doi]
PST - ppublish
SO  - Diabetes Res Clin Pract. 2008 Apr;80(1):63-8. doi: 10.1016/j.diabres.2007.10.023. 
      Epub 2008 Jan 22.