PMID- 18208975
OWN - NLM
STAT- MEDLINE
DCOM- 20080317
LR  - 20211020
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Print)
IS  - 0022-1007 (Linking)
VI  - 205
IP  - 2
DP  - 2008 Feb 18
TI  - iPLA2beta: front and center in human monocyte chemotaxis to MCP-1.
PG  - 347-59
LID - 10.1084/jem.20071243 [doi]
AB  - Monocyte chemoattractant protein-1 (MCP-1) directs migration of blood monocytes 
      to inflamed tissues. Despite the central role of chemotaxis in immune responses, 
      the regulation of chemotaxis by signal transduction pathways and their in vivo 
      significance remain to be thoroughly deciphered. In this study, we examined the 
      intracellular location and functions of two recently identified regulators of 
      chemotaxis, Ca(2+)-independent phospholipase (iPLA(2)beta) and cytosolic 
      phospholipase (cPLA(2)alpha), and substantiate their in vivo importance. These 
      enzymes are cytoplasmic in unstimulated monocytes. Upon MCP-1 stimulation, 
      iPLA(2)beta is recruited to the membrane-enriched pseudopod. In contrast, 
      cPLA(2)alpha is recruited to the endoplasmic reticulum. Although iPLA(2)beta or 
      cPLA(2)alpha antisense oligodeoxyribonucleotide (ODN)-treated monocytes display 
      reduced speed, iPLA(2)beta also regulates directionality and actin 
      polymerization. iPLA(2)beta or cPLA(2)alpha antisense ODN-treated adoptively 
      transferred mouse monocytes display a profound defect in migration to the 
      peritoneum in vivo. These converging observations reveal that iPLA(2)beta and 
      cPLA(2)alpha regulate monocyte migration from different intracellular locations, 
      with iPLA(2)beta acting as a critical regulator of the cellular compass, and 
      identify them as potential targets for antiinflammatory strategies.
FAU - Mishra, Ravi S
AU  - Mishra RS
AD  - Department of Cell Biology, Cleveland Clinic, Cleveland, OH 44195, USA.
FAU - Carnevale, Kevin A
AU  - Carnevale KA
FAU - Cathcart, Martha K
AU  - Cathcart MK
LA  - eng
GR  - M01-RR-018390/RR/NCRR NIH HHS/United States
GR  - M01 RR018390/RR/NCRR NIH HHS/United States
GR  - HL 61971/HL/NHLBI NIH HHS/United States
GR  - R01 HL061971/HL/NHLBI NIH HHS/United States
GR  - HL 510681/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20080121
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Actins)
RN  - 0 (Arachidonic Acids)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Naphthalenes)
RN  - 0 (Oligodeoxyribonucleotides, Antisense)
RN  - 0 (Pyrones)
RN  - 00XIW1CR0F (arachidonyltrifluoromethane)
RN  - 88070-98-8 (6-(bromomethylene)tetrahydro-3-(1-naphthaleneyl)-2H-pyran-2-one)
RN  - EC 3.1.1.4 (Group IV Phospholipases A2)
RN  - EC 3.1.1.4 (Group VI Phospholipases A2)
RN  - EC 3.1.1.4 (PLA2G6 protein, human)
SB  - IM
MH  - Actins/metabolism
MH  - Adoptive Transfer
MH  - Animals
MH  - Arachidonic Acids/pharmacology
MH  - Cells, Cultured
MH  - Chemokine CCL2/*immunology
MH  - Chemotaxis/drug effects/immunology
MH  - Female
MH  - Group IV Phospholipases A2/antagonists & inhibitors/*immunology
MH  - Group VI Phospholipases A2/antagonists & inhibitors/*immunology
MH  - Humans
MH  - Leukocytes, Mononuclear/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Monocytes/drug effects/*immunology
MH  - Naphthalenes/pharmacology
MH  - Oligodeoxyribonucleotides, Antisense/pharmacology
MH  - Peritonitis/chemically induced/immunology
MH  - Pyrones/pharmacology
PMC - PMC2271028
EDAT- 2008/01/23 09:00
MHDA- 2008/03/18 09:00
PMCR- 2008/08/18
CRDT- 2008/01/23 09:00
PHST- 2008/01/23 09:00 [pubmed]
PHST- 2008/03/18 09:00 [medline]
PHST- 2008/01/23 09:00 [entrez]
PHST- 2008/08/18 00:00 [pmc-release]
AID - jem.20071243 [pii]
AID - 20071243 [pii]
AID - 10.1084/jem.20071243 [doi]
PST - ppublish
SO  - J Exp Med. 2008 Feb 18;205(2):347-59. doi: 10.1084/jem.20071243. Epub 2008 Jan 
      21.