PMID- 18209476
OWN - NLM
STAT- MEDLINE
DCOM- 20080326
LR  - 20190212
IS  - 1015-8987 (Print)
IS  - 1015-8987 (Linking)
VI  - 21
IP  - 1-3
DP  - 2008
TI  - Decreased expression of Slc26a4 (Pendrin) and Slc26a7 in the kidneys of carbonic 
      anhydrase II-deficient mice.
PG  - 95-108
LID - 10.1159/000113751 [doi]
AB  - BACKGROUND/AIMS: Intercalated cells (ICs) of the kidney collecting duct are rich 
      in carbonic anhydrase II (CAII), which facilitates proton and bicarbonate 
      transport. Bicarbonate secretion is mediated via Pendrin (Slc26a4), which is 
      expressed on the apical membrane of B-ICs and nonA-nonB ICs in the cortical 
      collecting ducts (CCD). Bicarbonate absorption is mediated via anion exchanger 1 
      (AE1-Slc4a1) in the CCD and via AE1 and possibly Slc26a7 in the OMCD. Both 
      exchangers are expressed on the basolateral membrane of A-ICs. The aim of this 
      study was to examine the expression of pendrin, Slc26a7, and AE1 in the kidneys 
      of CAII-deficient (CAR2-null) mice. METHODS: For the expression studies, we used 
      real-time RT-PCR, Northern hybridization, immunolabeling, and immunoblotting. 
      RESULTS: Pendrin mRNA expression was reduced 63% along with decreased pendrin 
      immunolabeling in the cortex of CAR2-null mice present predominantly in nonA-nonB 
      ICs. Slc26a7 mRNA expression was decreases by 73% and Slc26a7 immunolabeling, 
      present in A-ICs, severely reduced in the outer medulla of CAR2-null mice. AE1 
      mRNA expression was decreased to a similar degree (62%) along with reduced AE1 
      immunolabeling. The expression of aquaporin 2 (AQP2) water channel, exclusively 
      present in principal cells of the collecting duct, was comparable in the wild 
      type and CAR2-null mice. CONCLUSION: CAII deficiency results in a significant 
      decrease in the gene and protein expression of bicarbonate transport proteins 
      from Slc26 gene family - Slc26a4 (pendrin) and Slc26a7. These results emphasize 
      the critical role of CAII for the maintenance of the intercalated cell phenotype.
FAU - Sun, Xuming
AU  - Sun X
AD  - Department of Medicine, University of Cincinnati, Cincinnati, OH 45267-0585, USA.
FAU - Soleimani, Manoocher
AU  - Soleimani M
FAU - Petrovic, Snezana
AU  - Petrovic S
LA  - eng
GR  - DK62809/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20080116
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Anion Exchange Protein 1, Erythrocyte)
RN  - 0 (Anion Transport Proteins)
RN  - 0 (Aquaporin 2)
RN  - 0 (Chloride-Bicarbonate Antiporters)
RN  - 0 (Slc26a4 protein, mouse)
RN  - 0 (Slc26a7 protein, mouse)
RN  - 0 (Slc4a1 protein, mouse)
RN  - 0 (Sulfate Transporters)
RN  - EC 3.6.3.14 (Proton-Translocating ATPases)
RN  - EC 4.2.1.- (Carbonic Anhydrase II)
SB  - IM
MH  - Animals
MH  - Anion Exchange Protein 1, Erythrocyte/genetics/metabolism
MH  - Anion Transport Proteins/*genetics/*metabolism
MH  - Aquaporin 2/genetics/metabolism
MH  - Blotting, Western
MH  - Carbonic Anhydrase II/*deficiency
MH  - Chloride-Bicarbonate Antiporters/*genetics/*metabolism
MH  - Densitometry
MH  - Gene Expression Regulation
MH  - Kidney/cytology/*enzymology
MH  - Mice
MH  - Proton-Translocating ATPases/metabolism
MH  - Sulfate Transporters
EDAT- 2008/01/23 09:00
MHDA- 2008/03/28 09:00
CRDT- 2008/01/23 09:00
PHST- 2007/11/15 00:00 [accepted]
PHST- 2008/01/23 09:00 [pubmed]
PHST- 2008/03/28 09:00 [medline]
PHST- 2008/01/23 09:00 [entrez]
AID - 000113751 [pii]
AID - 10.1159/000113751 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2008;21(1-3):95-108. doi: 10.1159/000113751. Epub 2008 Jan 
      16.