PMID- 18215246 OWN - NLM STAT- MEDLINE DCOM- 20080307 LR - 20240109 IS - 1601-5223 (Electronic) IS - 0018-0661 (Linking) VI - 144 IP - 6 DP - 2007 Dec TI - Chromosomal study of lettuce and its allied species (Lactuca spp., Asteraceae) by means of karyotype analysis and fluorescence in situ hybridization. PG - 235-43 LID - 10.1111/j.2007.0018-0661.02012x [doi] AB - In this study, in addition to the karyotype analysis, the chromosomal distributions of 5 S and 18 S rDNAs, and the Arabidopsis-type (T3AG3) telomeric sequences were detected by means of fluorescence in situ hybridization (FISH) to promote the information of chromosomal organization and evolution in the cultivated lettuce and its wild relatives, L. sativa, L. serriola, L. saligna and L. virosa. The karyotype analysis revealed the dissimilarity between L. virosa and the remaining species. In all four Lactuca species studied, one 5 S rDNA and two 18 S rDNA loci were detected. The simultaneous FISH of 5 S and 18 S rDNAs revealed that both rDNA loci of L. sativa, L. serriola and L. saligna were identical, however, that of L. virosa was different from the other species. These analyses indicate the closer relationships between L. sativa/L. serriola and L. saligna rather than L. virosa. Arabidopsis-type telomeric sequences were detected at both ends of their chromatids of all chromosomes not in the other regions. This observation suggests the lack of telomere-mediated chromosomal rearrangements among the Lactuca chromosomes. FAU - Matoba, Hideyuki AU - Matoba H AD - Department of Applied Biological Science, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan. FAU - Mizutani, Takayuki AU - Mizutani T FAU - Nagano, Katsuya AU - Nagano K FAU - Hoshi, Yoshikazu AU - Hoshi Y FAU - Uchiyama, Hiroshi AU - Uchiyama H LA - eng PT - Journal Article PL - England TA - Hereditas JT - Hereditas JID - 0374654 RN - 0 (DNA, Ribosomal) SB - IM MH - Arabidopsis/genetics MH - Chromosome Mapping/methods MH - Chromosomes, Plant MH - DNA, Ribosomal/genetics MH - *Genes, Plant MH - Genetic Techniques MH - Hybridization, Genetic MH - In Situ Hybridization, Fluorescence/*methods MH - Karyotyping MH - Lactuca/*genetics MH - Metaphase MH - Models, Genetic MH - Nucleic Acid Hybridization MH - Polymerase Chain Reaction EDAT- 2008/01/25 09:00 MHDA- 2008/03/08 09:00 CRDT- 2008/01/25 09:00 PHST- 2008/01/25 09:00 [pubmed] PHST- 2008/03/08 09:00 [medline] PHST- 2008/01/25 09:00 [entrez] AID - HRD2012 [pii] AID - 10.1111/j.2007.0018-0661.02012x [doi] PST - ppublish SO - Hereditas. 2007 Dec;144(6):235-43. doi: 10.1111/j.2007.0018-0661.02012x.