PMID- 18216291
OWN - NLM
STAT- MEDLINE
DCOM- 20080728
LR  - 20171116
IS  - 1460-2407 (Electronic)
IS  - 1360-9947 (Linking)
VI  - 14
IP  - 3
DP  - 2008 Mar
TI  - Progesterone receptor modulator CDB-2914 induces extracellular matrix 
      metalloproteinase inducer in cultured human uterine leiomyoma cells.
PG  - 181-91
LID - 10.1093/molehr/gan004 [doi]
AB  - Effects of progesterone receptor modulator CDB-2914 on the expression of the 
      extracellular matrix (ECM) components were examined in cultured human uterine 
      leiomyoma and myometrial cells. ECM metalloproteinase inducer (EMMPRIN), matrix 
      metalloproteinases (MMPs), tissue inhibitors of MMP (TIMPs) and collagen levels 
      were assessed by Western blot analysis, MMP activity assay and real-time RT-PCR. 
      RNA interference (RNAi) of EMMPRIN was performed using small interfering mRNA. In 
      cultured leiomyoma cells, CDB-2914 treatment at concentrations greater than or 
      equal to 10(-8) M significantly increased EMMPRIN, MMP-1 and MMP-8 protein 
      contents and MMP-1, MMP-2, MMP-3 and MMP-9 mRNA levels, and activity of MMP-1, 
      MMP-2, MMP-3 and MMP-9 in the medium. TIMP-1 and TIMP-2 were significantly 
      decreased at mRNA and protein levels by CDB-2914 treatment at concentrations > or 
      =10(-7) M in these cells. CDB-2914 treatment decreased types I and III collagen 
      protein contents. However, CDB-2914 treatment did not affect the ECM component 
      expression in cultured myometrial cells. RNAi of EMMPRIN abrogated 
      CDB-2914-mediated both induction of MMPs and reduction of TIMPs and collagens in 
      cultured leiomyoma cells. These results suggest that CDB-2914 modulates the 
      expression of EMMPRIN, MMPs, TIMPs and collagens in cultured leiomyoma cells 
      without comparable effects on myometrial cells.
FAU - Xu, Qin
AU  - Xu Q
AD  - Department of Obstetrics and Gynecology, Kobe University Graduate School of 
      Medicine, 7-5-1 Kusunoki-Cho, Chuo-Ku, Kobe, Hyogo Prefecture 650-0017, Japan.
FAU - Ohara, Noriyuki
AU  - Ohara N
FAU - Liu, Jin
AU  - Liu J
FAU - Amano, Mariko
AU  - Amano M
FAU - Sitruk-Ware, Regine
AU  - Sitruk-Ware R
FAU - Yoshida, Shigeki
AU  - Yoshida S
FAU - Maruo, Takeshi
AU  - Maruo T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080123
PL  - England
TA  - Mol Hum Reprod
JT  - Molecular human reproduction
JID - 9513710
RN  - 0 (BSG protein, human)
RN  - 0 (Norpregnadienes)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, Progesterone)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - 136894-56-9 (Basigin)
RN  - 6J5J15Q2X8 (ulipristal)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Adult
MH  - Basigin/genetics/metabolism
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Female
MH  - Humans
MH  - Leiomyoma/genetics/metabolism/pathology
MH  - Matrix Metalloproteinases/genetics/*metabolism
MH  - Middle Aged
MH  - Myometrium/drug effects/metabolism
MH  - Norpregnadienes/*pharmacology
MH  - RNA, Small Interfering/genetics
MH  - Receptors, Progesterone/*antagonists & inhibitors
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tissue Inhibitor of Metalloproteinase-1/genetics/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-2/genetics/metabolism
MH  - Tumor Cells, Cultured
MH  - Uterine Neoplasms/metabolism/pathology
EDAT- 2008/01/25 09:00
MHDA- 2008/07/29 09:00
CRDT- 2008/01/25 09:00
PHST- 2008/01/25 09:00 [pubmed]
PHST- 2008/07/29 09:00 [medline]
PHST- 2008/01/25 09:00 [entrez]
AID - gan004 [pii]
AID - 10.1093/molehr/gan004 [doi]
PST - ppublish
SO  - Mol Hum Reprod. 2008 Mar;14(3):181-91. doi: 10.1093/molehr/gan004. Epub 2008 Jan 
      23.