PMID- 18217951
OWN - NLM
STAT- MEDLINE
DCOM- 20080609
LR  - 20211020
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Print)
IS  - 0019-2805 (Linking)
VI  - 124
IP  - 2
DP  - 2008 Jun
TI  - Effect of SIVmac infection on plasmacytoid and CD1c+ myeloid dendritic cells in 
      cynomolgus macaques.
PG  - 223-33
LID - 10.1111/j.1365-2567.2007.02758.x [doi]
AB  - Dendritic cells (DCs) are known to be essential for the induction and regulation 
      of immune responses. Non-human primates are essential in biomedical research and 
      contribute to our understanding of the involvement of DCs in human infectious 
      diseases. However, no direct single-platform method for quantifying DC precursors 
      has yet been optimized in macaques to give accurate absolute blood counts of 
      these rare-event cell populations in the blood. We adapted a rapid whole-blood 
      assay for the absolute quantification of DCs in cynomolgus macaques by 
      four-colour flow cytometry, using a single-platform assay compatible with human 
      blood. Cynomolgus macaque plasmacytoid DCs (pDCs) and CD1c(+) myeloid DCs 
      (CD1c(+) mDCs) were quantified in the blood of 34 healthy macaques and the 
      results obtained were compared with those for blood samples from 11 healthy 
      humans. In addition, circulating absolute numbers of pDCs were quantified in 
      cynomolgus macaques chronically infected with SIVmac. During infection, pDC 
      counts decreased whereas circulating CD1c(+) mDC counts increased. Information 
      regarding absolute pDC and mDC counts in non-human primates may improve our 
      understanding of the role of these cells in SIV/HIV infection and in other 
      infectious diseases.
FAU - Malleret, Benoit
AU  - Malleret B
AD  - CEA, Service d'Immuno-Virologie, DSV/iMETI, IPSC, Fontenay-aux-Roses, France.
FAU - Karlsson, Ingrid
AU  - Karlsson I
FAU - Maneglier, Benjamin
AU  - Maneglier B
FAU - Brochard, Patricia
AU  - Brochard P
FAU - Delache, Benoit
AU  - Delache B
FAU - Andrieu, Thibault
AU  - Andrieu T
FAU - Muller-Trutwin, Michaela
AU  - Muller-Trutwin M
FAU - Beaumont, Tim
AU  - Beaumont T
FAU - McCune, Joseph M
AU  - McCune JM
FAU - Banchereau, Jacques
AU  - Banchereau J
FAU - Le Grand, Roger
AU  - Le Grand R
FAU - Vaslin, Bruno
AU  - Vaslin B
LA  - eng
GR  - R37 AI040312/AI/NIAID NIH HHS/United States
GR  - U19 AI057234/AI/NIAID NIH HHS/United States
GR  - 5U19AI-057234/AI/NIAID NIH HHS/United States
GR  - R37 AI40312/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080124
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antigens, CD1)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Antigens, CD1/*blood
MH  - Cell Count
MH  - Chronic Disease
MH  - Dendritic Cells/*immunology
MH  - Flow Cytometry
MH  - Humans
MH  - Immunophenotyping
MH  - Lymph Nodes/immunology
MH  - Macaca fascicularis
MH  - Male
MH  - Myeloid Progenitor Cells/*immunology
MH  - Simian Acquired Immunodeficiency Syndrome/*immunology
MH  - *Simian Immunodeficiency Virus
PMC - PMC2566627
EDAT- 2008/01/26 09:00
MHDA- 2008/06/10 09:00
PMCR- 2009/06/01
CRDT- 2008/01/26 09:00
PHST- 2008/01/26 09:00 [pubmed]
PHST- 2008/06/10 09:00 [medline]
PHST- 2008/01/26 09:00 [entrez]
PHST- 2009/06/01 00:00 [pmc-release]
AID - IMM2758 [pii]
AID - 10.1111/j.1365-2567.2007.02758.x [doi]
PST - ppublish
SO  - Immunology. 2008 Jun;124(2):223-33. doi: 10.1111/j.1365-2567.2007.02758.x. Epub 
      2008 Jan 24.