PMID- 18219282
OWN - NLM
STAT- MEDLINE
DCOM- 20080422
LR  - 20191210
IS  - 1751-7362 (Print)
IS  - 1751-7362 (Linking)
VI  - 2
IP  - 3
DP  - 2008 Mar
TI  - 3D FISH for the quantification of methane- and sulphur-oxidizing endosymbionts in 
      bacteriocytes of the hydrothermal vent mussel Bathymodiolus azoricus.
PG  - 284-92
LID - 10.1038/ismej.2008.3 [doi]
AB  - Dual endosymbioses involving methane- and sulphur-oxidizing bacteria occur in the 
      gills of several species of mussels from deep-sea hydrothermal vents and cold 
      seeps. Variations of total and relative abundances of symbionts depending on 
      local environmental parameters are not yet understood, due to a lack of reliable 
      quantification of bacteria in the host tissue. Here, we report the first attempt 
      to quantify volumes occupied by each type of symbiont in bacteriocyte sections 
      from a vent mussel, Bathymodiolus azoricus, using fluorescence in situ 
      hybridization (FISH) coupled to three dimentional microscopy and image analysis 
      carried out by a dedicated software, which we developed. Bacteriocytes from 
      mussels recovered at different vent sites displayed significantly different 
      abundances of bacteria. Specimens kept in aquaria at atmospheric pressure and 
      exposed to an artificial pulse of sulphur displayed an increase in absolute and 
      relative abundance of sulphur oxidizers within their bacteriocytes. Distributions 
      of all measured parameters fitted normal distributions, indicating that 
      bacteriocytes from a specimen tend to display similar behaviours. This study 
      shows that symbiont volume quantification is tractable using 3D FISH, and 
      confirms the impact of local environmental parameters on symbiont abundances.
FAU - Halary, Sebastien
AU  - Halary S
AD  - UPMC Univ Paris 06, UMR 7138 Systematique Adaptation Evolution, equipe Adaptation 
      aux Milieux Extremes, Paris, France.
FAU - Riou, Virginie
AU  - Riou V
FAU - Gaill, Francoise
AU  - Gaill F
FAU - Boudier, Thomas
AU  - Boudier T
FAU - Duperron, Sebastien
AU  - Duperron S
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080124
PL  - England
TA  - ISME J
JT  - The ISME journal
JID - 101301086
RN  - 70FD1KFU70 (Sulfur)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Animals
MH  - Bacteria/genetics/*isolation & purification
MH  - Bacteriological Techniques
MH  - Epithelial Cells/*microbiology
MH  - Gills/cytology/*microbiology
MH  - Imaging, Three-Dimensional/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Methane/metabolism
MH  - Mytilidae/*microbiology
MH  - Oxidation-Reduction
MH  - Seawater
MH  - Sulfur/metabolism
MH  - Symbiosis
EDAT- 2008/01/26 09:00
MHDA- 2008/04/23 09:00
CRDT- 2008/01/26 09:00
PHST- 2008/01/26 09:00 [pubmed]
PHST- 2008/04/23 09:00 [medline]
PHST- 2008/01/26 09:00 [entrez]
AID - ismej20083 [pii]
AID - 10.1038/ismej.2008.3 [doi]
PST - ppublish
SO  - ISME J. 2008 Mar;2(3):284-92. doi: 10.1038/ismej.2008.3. Epub 2008 Jan 24.