PMID- 18219580
OWN - NLM
STAT- MEDLINE
DCOM- 20090209
LR  - 20131121
IS  - 1573-6822 (Electronic)
IS  - 0742-2091 (Linking)
VI  - 24
IP  - 6
DP  - 2008 Dec
TI  - Echinoderm regenerative response as a sensitive ecotoxicological test for the 
      exposure to endocrine disrupters: effects of p,p'DDE and CPA on crinoid arm 
      regeneration.
PG  - 573-86
LID - 10.1007/s10565-008-9057-y [doi]
AB  - Echinoderms are valuable test species in marine ecotoxicology and offer a wide 
      range of biological processes appropriate for this approach. Regenerating 
      echinoderms can be regarded as amenable experimental models for testing the 
      effects of exposure to contaminants, particularly endocrine disrupter compounds 
      (EDCs). As regeneration is a typical developmental process, physiologically 
      regulated by humoral mechanisms, it is highly susceptible to the action of 
      pseudo-hormonal contaminants which appear to be obvious candidates for exerting 
      deleterious actions. In our laboratory experiments, selected EDCs suspected for 
      their antiandrogenic action (p,p'-DDE and cyproterone acetate) were tested at low 
      concentrations on regenerating specimens of the crinoid Antedon mediterranea. An 
      integrated approach which combines exposure experiments and different 
      morphological analyses was employed; the obtained results suggest an overall 
      pattern of plausible endocrine disruption in the exposed samples, showing that 
      processes such as regenerative growth, histogenesis, and differentiation are 
      affected by the exposure to the selected compounds. These results confirm that 
      (1) regenerative phenomena of echinoderms can be considered valuable alternative 
      models to assess the effects of exposure to exogenous substances such as EDCs, 
      and (2) these compounds significantly interfere with fundamental processes of 
      developmental physiology (proliferation, differentiation, etc...) plausibly via 
      endocrine alterations. In terms of future prospects, taking into account the 
      increasing need to propose animal models different from vertebrates, echinoderms 
      represent a group on which ecotoxicological studies should be encouraged and 
      specifically addressed.
FAU - Sugni, Michela
AU  - Sugni M
AD  - Dipartimento di Biologia, Universita degli Studi di Milano, Milan, Italy. 
      michela.sugni@unimi.it
FAU - Manno, Valentina
AU  - Manno V
FAU - Barbaglio, Alice
AU  - Barbaglio A
FAU - Mozzi, Daniela
AU  - Mozzi D
FAU - Bonasoro, Francesco
AU  - Bonasoro F
FAU - Tremolada, Paolo
AU  - Tremolada P
FAU - Candia Carnevali, M Daniela
AU  - Candia Carnevali MD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080125
PL  - Switzerland
TA  - Cell Biol Toxicol
JT  - Cell biology and toxicology
JID - 8506639
RN  - 0 (Endocrine Disruptors)
RN  - 4KM2BN5JHF (Cyproterone Acetate)
RN  - V14159DF29 (Dichlorodiphenyldichloroethane)
SB  - IM
MH  - Animals
MH  - Cell Proliferation/drug effects
MH  - Cyproterone Acetate/*toxicity
MH  - Dichlorodiphenyldichloroethane/*toxicity
MH  - Echinodermata/cytology/*drug effects/physiology
MH  - Endocrine Disruptors/*toxicity
MH  - Environmental Exposure
MH  - Extremities/*physiology
MH  - Regeneration/*drug effects
MH  - *Toxicity Tests
EDAT- 2008/01/26 09:00
MHDA- 2009/02/10 09:00
CRDT- 2008/01/26 09:00
PHST- 2007/10/29 00:00 [received]
PHST- 2008/01/03 00:00 [accepted]
PHST- 2008/01/26 09:00 [pubmed]
PHST- 2009/02/10 09:00 [medline]
PHST- 2008/01/26 09:00 [entrez]
AID - 10.1007/s10565-008-9057-y [doi]
PST - ppublish
SO  - Cell Biol Toxicol. 2008 Dec;24(6):573-86. doi: 10.1007/s10565-008-9057-y. Epub 
      2008 Jan 25.