PMID- 18220258
OWN - NLM
STAT- MEDLINE
DCOM- 20080318
LR  - 20220317
IS  - 1096-9861 (Electronic)
IS  - 0021-9967 (Linking)
VI  - 507
IP  - 4
DP  - 2008 Apr 1
TI  - Deafferentation-associated changes in afferent and efferent processes in the 
      guinea pig cochlea and afferent regeneration with chronic intrascalar 
      brain-derived neurotrophic factor and acidic fibroblast growth factor.
PG  - 1602-21
LID - 10.1002/cne.21619 [doi]
AB  - Deafferentation of the auditory nerve from loss of sensory cells is associated 
      with degeneration of nerve fibers and spiral ganglion neurons (SGN). SGN survival 
      following deafferentation can be enhanced by application of neurotrophic factors 
      (NTF), and NTF can induce the regrowth of SGN peripheral processes. Cochlear 
      prostheses could provide targets for regrowth of afferent peripheral processes, 
      enhancing neural integration of the implant, decreasing stimulation thresholds, 
      and increasing specificity of stimulation. The present study analyzed 
      distribution of afferent and efferent nerve fibers following deafness in guinea 
      pigs using specific markers (parvalbumin for afferents, synaptophysin for 
      efferent fibers) and the effect of brain derived neurotrophic factor (BDNF) in 
      combination with acidic fibroblast growth factor (aFGF). Immediate treatment 
      following deafness was compared with 3-week-delayed NTF treatment. Histology of 
      the cochlea with immunohistochemical techniques allowed quantitative analysis of 
      neuron and axonal changes. Effects of NTF were assessed at the light and electron 
      microscopic levels. Chronic BDNF/aFGF resulted in a significantly increased 
      number of afferent peripheral processes in both immediate- and delayed-treatment 
      groups. Outgrowth of afferent nerve fibers into the scala tympani were observed, 
      and SGN densities were found to be higher than in normal hearing animals. These 
      new SGN might have developed from endogenous progenitor/stem cells, recently 
      reported in human and mouse cochlea, under these experimental conditions of 
      deafferentation-induced stress and NTF treatment. NTF treatment provided no 
      enhanced maintenance of efferent fibers, although some synaptophysin-positive 
      fibers were detected at atypical sites, suggesting some sprouting of efferent 
      fibers.
CI  - Copyright 2008 Wiley-Liss, Inc.
FAU - Glueckert, Rudolf
AU  - Glueckert R
AD  - Department of Otolaryngology, Medical University of Innsbruck, A-6020 Innsbruck, 
      Austria.
FAU - Bitsche, Mario
AU  - Bitsche M
FAU - Miller, Josef M
AU  - Miller JM
FAU - Zhu, Yaying
AU  - Zhu Y
FAU - Prieskorn, Diane M
AU  - Prieskorn DM
FAU - Altschuler, Richard A
AU  - Altschuler RA
FAU - Schrott-Fischer, Anneliese
AU  - Schrott-Fischer A
LA  - eng
GR  - P30 DC005188/DC/NIDCD NIH HHS/United States
GR  - R01 DC003820/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Parvalbumins)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (Synaptophysin)
RN  - 104781-85-3 (Fibroblast Growth Factor 1)
RN  - 59-01-8 (Kanamycin)
RN  - M5DP350VZV (Ethacrynic Acid)
SB  - IM
MH  - Animals
MH  - Auditory Pathways/drug effects/pathology
MH  - Brain-Derived Neurotrophic Factor/administration & dosage
MH  - Cochlea/*drug effects/pathology
MH  - Deafness/chemically induced/drug therapy/*pathology
MH  - Enzyme Inhibitors/toxicity
MH  - Ethacrynic Acid/toxicity
MH  - Female
MH  - Fibroblast Growth Factor 1/administration & dosage
MH  - Guinea Pigs
MH  - Image Processing, Computer-Assisted
MH  - Immunohistochemistry
MH  - Intercellular Signaling Peptides and Proteins/*administration & dosage
MH  - Kanamycin/toxicity
MH  - Male
MH  - Microscopy, Confocal
MH  - Microscopy, Electron, Transmission
MH  - Nerve Regeneration/*drug effects
MH  - Neurons/drug effects/pathology
MH  - Neurons, Afferent/drug effects/*pathology
MH  - Neurons, Efferent/drug effects/*pathology
MH  - Parvalbumins/metabolism
MH  - Protein Synthesis Inhibitors/toxicity
MH  - Synaptophysin/metabolism
MH  - Time
EDAT- 2008/01/29 09:00
MHDA- 2008/03/19 09:00
CRDT- 2008/01/29 09:00
PHST- 2008/01/29 09:00 [pubmed]
PHST- 2008/03/19 09:00 [medline]
PHST- 2008/01/29 09:00 [entrez]
AID - 10.1002/cne.21619 [doi]
PST - ppublish
SO  - J Comp Neurol. 2008 Apr 1;507(4):1602-21. doi: 10.1002/cne.21619.