PMID- 18221884
OWN - NLM
STAT- MEDLINE
DCOM- 20080415
LR  - 20171116
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 41
IP  - 3
DP  - 2008 Mar
TI  - A comparative analysis of cytokine responses, cell surface marker expression and 
      MAPKs in DCs matured with LPS compared with a panel of TLR ligands.
PG  - 254-62
LID - 10.1016/j.cyto.2007.11.020 [doi]
AB  - Dendritic cells (DCs) are professional antigen-presenting cells that play a vital 
      role in shaping adaptive immunity. DC maturation begins when exogenous danger 
      signals bind to the appropriate toll-like receptor (TLR) and initiate expression 
      of cell surface markers and the secretion of cytokines. This process occurs 
      through defined mitogen-activated protein kinase (MAPK) signalling pathways. Of 
      the 13 known mammalian TLRs, lipopolysaccharide (LPS), which activates TLR4, is 
      the most commonly used ligand for the maturation of DCs in vitro. This 
      comprehensive study measures cytokine secretion and cell surface marker 
      expression in murine bone-marrow-derived DCs following maturation with LPS 
      compared to DCs matured with a panel of other TLR-ligands (zymosan A (TLR2/6), 
      PGN (TLR2), poly(I:C) (TLR3), flagellin (TLR5) and CpG-ODN1826 (TLR9)). The role 
      of MAPK signalling pathways in the maturation process was also examined. Results 
      demonstrate that zymosan A and CpG induce comparable cytokine and cell surface 
      marker profiles to LPS. The remaining ligands differed significantly for cytokine 
      and CD40 expression, but not for CD80 and CD86 expression. While there were 
      differences for MAPK signalling pathways for all ligands, the effect of the 
      inhibitors were broadly similar. These findings broaden our knowledge of TLR 
      ligand-matured DCs.
FAU - Dowling, David
AU  - Dowling D
AD  - Parasite Immune Modulation Group, School of Nursing, Faculty of Science and 
      Health, Dublin City University, Dublin, Ireland.
FAU - Hamilton, Clare M
AU  - Hamilton CM
FAU - O'Neill, Sandra M
AU  - O'Neill SM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080124
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Antigens, CD)
RN  - 0 (CpG ODN 1826)
RN  - 0 (Cytokines)
RN  - 0 (Ligands)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Toll-Like Receptors)
RN  - 12777-81-0 (Flagellin)
RN  - 9007-49-2 (DNA)
RN  - 9010-72-4 (Zymosan)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - Animals
MH  - Antigens, CD/analysis/metabolism
MH  - Cytokines/*metabolism
MH  - DNA/analysis/immunology
MH  - Dendritic Cells/drug effects/*immunology
MH  - Female
MH  - Flagellin/immunology/pharmacology
MH  - Ligands
MH  - Lipopolysaccharides/*immunology/pharmacology
MH  - Mice
MH  - Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism
MH  - Oligodeoxyribonucleotides/immunology/pharmacology
MH  - Poly I-C/immunology/pharmacology
MH  - Toll-Like Receptors/*agonists
MH  - Zymosan/immunology/pharmacology
EDAT- 2008/01/29 09:00
MHDA- 2008/04/16 09:00
CRDT- 2008/01/29 09:00
PHST- 2007/09/24 00:00 [received]
PHST- 2007/11/27 00:00 [revised]
PHST- 2007/11/30 00:00 [accepted]
PHST- 2008/01/29 09:00 [pubmed]
PHST- 2008/04/16 09:00 [medline]
PHST- 2008/01/29 09:00 [entrez]
AID - S1043-4666(07)00517-0 [pii]
AID - 10.1016/j.cyto.2007.11.020 [doi]
PST - ppublish
SO  - Cytokine. 2008 Mar;41(3):254-62. doi: 10.1016/j.cyto.2007.11.020. Epub 2008 Jan 
      24.