PMID- 18222913
OWN - NLM
STAT- MEDLINE
DCOM- 20081020
LR  - 20220309
IS  - 1939-4640 (Electronic)
IS  - 0196-3635 (Linking)
VI  - 29
IP  - 3
DP  - 2008 May-Jun
TI  - Altered expression of muscle- and cytoskeleton-related genes in a rat strain with 
      inherited cryptorchidism.
PG  - 352-66
LID - 10.2164/jandrol.107.003970 [doi]
AB  - Development of the fetal gubernaculum is a prerequisite for testicular descent 
      and dependent on insulin-like 3 and androgen, but knowledge of downstream 
      effectors is limited. We analyzed transcript profiles in gubernaculum and testis 
      to address changes occurring during normal and abnormal testicular descent in 
      Long Evans wild-type (wt) and cryptorchid (orl) fetuses. Total RNA from male wt 
      and orl gubernacula (gestational days [GD]18-20), wt female gubernacula (GD18), 
      and testis (GD17 and 19) was hybridized to Affymetrix GeneChips. Statistical 
      analysis of temporal, gender, and strain-specific differences in gene expression 
      was performed with the use of linear models analysis with empirical Bayes 
      statistics and analysis of variance (gubernaculum) and linear analysis (testis). 
      Overrepresented common gene ontology functional categories and pathways were 
      identified in groups of differentially expressed genes with the Database for 
      Annotation, Visualization, and Integrated Discovery. Transcript profiles were 
      dynamic in wt males between GD18-19 and GD20, comparatively static in orl GD18-20 
      gubernaculum, and similar in wt and orl testis. Functional analysis of 
      differentially expressed genes in wt and orl gubernaculum identified categories 
      related to metabolism, cellular biogenesis, small GTPase-mediated signal 
      transduction, cytoskeleton, muscle development, and insulin signaling. Genes 
      involved in androgen receptor signaling, regulated by androgens, or both were 
      overrepresented in differentially expressed gubernaculum and testis gene groups. 
      Quantitative reverse transcription polymerase chain reaction (RT-PCR) confirmed 
      differential expression of genes related to muscle development, including Myog, 
      Tnnt2, Fst, Igf1, Igfbp5, Id2, and Msx1. These data suggest that the orl mutation 
      results in a primary gubernacular defect that affects muscle development and 
      cytoskeletal function and might alter androgen-regulated pathways.
FAU - Barthold, Julia S
AU  - Barthold JS
AD  - Nemours Biomedical Research and Division of Urology, A.I. duPont Hospital for 
      Children, Wilmington, Delaware 19803, USA. jbarthol@nemours.org
FAU - McCahan, Suzanne M
AU  - McCahan SM
FAU - Singh, Amar V
AU  - Singh AV
FAU - Knudsen, Thomas B
AU  - Knudsen TB
FAU - Si, Xiaoli
AU  - Si X
FAU - Campion, Liam
AU  - Campion L
FAU - Akins, Robert E
AU  - Akins RE
LA  - eng
GR  - P20 RR020173/RR/NCRR NIH HHS/United States
GR  - P20 RR-020173-01/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20080124
PL  - United States
TA  - J Androl
JT  - Journal of andrology
JID - 8106453
RN  - EC 3.6.5.2 (Monomeric GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Cryptorchidism/*genetics/metabolism
MH  - Cytoskeleton/*genetics/metabolism
MH  - Energy Metabolism/genetics
MH  - Fetal Development/physiology
MH  - Gene Expression
MH  - Gene Expression Profiling
MH  - Male
MH  - Monomeric GTP-Binding Proteins/metabolism
MH  - Muscle Development/*genetics
MH  - Oligonucleotide Array Sequence Analysis
MH  - Rats
MH  - Signal Transduction/physiology
MH  - Testis/*growth & development/metabolism
EDAT- 2008/01/29 09:00
MHDA- 2008/10/22 09:00
CRDT- 2008/01/29 09:00
PHST- 2008/01/29 09:00 [pubmed]
PHST- 2008/10/22 09:00 [medline]
PHST- 2008/01/29 09:00 [entrez]
AID - jandrol.107.003970 [pii]
AID - 10.2164/jandrol.107.003970 [doi]
PST - ppublish
SO  - J Androl. 2008 May-Jun;29(3):352-66. doi: 10.2164/jandrol.107.003970. Epub 2008 
      Jan 24.