PMID- 18222995
OWN - NLM
STAT- MEDLINE
DCOM- 20080519
LR  - 20211020
IS  - 1542-0086 (Electronic)
IS  - 0006-3495 (Print)
IS  - 0006-3495 (Linking)
VI  - 94
IP  - 9
DP  - 2008 May 1
TI  - Electronic wiring of a multi-redox site membrane protein in a biomimetic surface 
      architecture.
PG  - 3698-705
LID - 10.1529/biophysj.107.117846 [doi]
AB  - Bioelectronic coupling of multi-redox-site membrane proteins was accomplished 
      with cytochrome c oxidase (CcO) as an example. A biomimetic membrane system was 
      used for the oriented immobilization of the CcO oxidase on a metal electrode. 
      When the protein is immobilized with the CcO binding side directed toward the 
      electrode and reconstituted in situ into a lipid bilayer, it is addressable by 
      direct electron transfer to the redox centers. Electron transfer to the enzyme 
      via the spacer, referred to as electronic wiring, shows an exceptionally high 
      rate constant. This allows a kinetic analysis of all four consecutive electron 
      transfer steps within the enzyme to be carried out. Electron transfer followed by 
      rapid scan cyclic voltammetry in combination with surface-enhanced resonance 
      Raman spectroscopy provides mechanistic and structural information about the heme 
      centers. Probing the enzyme under turnover conditions showed mechanistic insights 
      into proton translocation coupled to electron transfer. This bioelectronic 
      approach opens a new field of activity to investigate complex processes in a wide 
      variety of membrane proteins.
FAU - Friedrich, Marcel G
AU  - Friedrich MG
AD  - Max Planck Institute for Polymer Research, Mainz, Germany.
FAU - Robertson, Joseph W F
AU  - Robertson JW
FAU - Walz, Dieter
AU  - Walz D
FAU - Knoll, Wolfgang
AU  - Knoll W
FAU - Naumann, Renate L C
AU  - Naumann RL
LA  - eng
PT  - Journal Article
DEP - 20080125
PL  - United States
TA  - Biophys J
JT  - Biophysical journal
JID - 0370626
RN  - 0 (Protons)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Anaerobiosis
MH  - Biomimetic Materials/*metabolism
MH  - Electrochemistry
MH  - Electrodes
MH  - Electron Transport
MH  - Electron Transport Complex IV/*metabolism
MH  - Ion Transport
MH  - Kinetics
MH  - Protons
MH  - Rhodobacter sphaeroides/*enzymology
PMC - PMC2292391
EDAT- 2008/01/29 09:00
MHDA- 2008/05/20 09:00
PMCR- 2009/05/01
CRDT- 2008/01/29 09:00
PHST- 2008/01/29 09:00 [pubmed]
PHST- 2008/05/20 09:00 [medline]
PHST- 2008/01/29 09:00 [entrez]
PHST- 2009/05/01 00:00 [pmc-release]
AID - S0006-3495(08)70445-4 [pii]
AID - 117846 [pii]
AID - 10.1529/biophysj.107.117846 [doi]
PST - ppublish
SO  - Biophys J. 2008 May 1;94(9):3698-705. doi: 10.1529/biophysj.107.117846. Epub 2008 
      Jan 25.