PMID- 18225390
OWN - NLM
STAT- MEDLINE
DCOM- 20080212
LR  - 20161020
IS  - 0884-6812 (Linking)
VI  - 29
IP  - 6
DP  - 2007 Dec
TI  - Fluorescence in situ hybridization determination of aneusomy: criteria and 
      technical considerations.
PG  - 351-7
AB  - OBJECTIVE: To investigate a series of tissues to determine if proliferation rate 
      can affect chromosome counts by fluorescence in situ hybridization (FISH). STUDY 
      DESIGN: We studied 9 non-neoplastic tissues and a trisomy 7 and tetrasomy 13 cell 
      line by FISH. For each sample, 100 cells were analyzed for chromosome 7 and 13 
      number and MIB-1 expression. Centrometric enumeration probe (CEP) 7 counts were 
      correlated with proliferation index. RESULTS: Average CEP 7 number showed a 
      relationship to proliferation index, with higher CEP 7 averages associated with 
      higher proliferation indices. Specimens of brain tissue demonstrated average CEP 
      7 counts between 1.64 and 1.75. Tissues with high proliferation indices (23-66%) 
      demonstrated CEP 7 counts between 2.14 and 2.31. The average CEP 7 count for the 
      trisomy 7 cell line was 2.61. The average LSI 13 count for the tetrasomy 13 cell 
      line was 3.65. CONCLUSION: Chromosome 7 FISH counts demonstrated overlap between 
      diploid tissues with high proliferation and triploid chromosome 7 tissues. This 
      overlap was seen when 95% CI limits were used. The trisomic 7 and tetrasomic 13 
      cell lines demonstrated average CEP 7 and CEP 13 levels below 3 and 4, 
      respectively. Definitions used for determination of polysomy should take into 
      account tissue proliferation and section thicknesses.
FAU - Chadwick, Barbara
AU  - Chadwick B
AD  - Department of Pathology, University of Utah School of Medicine, 50 North Medical 
      Drive, Salt Lake City, Utah 84132, USA.
FAU - Willmore-Payne, Carlynn
AU  - Willmore-Payne C
FAU - Holden, Joseph
AU  - Holden J
FAU - Layfield, Lester
AU  - Layfield L
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Anal Quant Cytol Histol
JT  - Analytical and quantitative cytology and histology
JID - 8506819
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (MIB-1 antibody)
SB  - IM
MH  - *Aneuploidy
MH  - Antibodies, Antinuclear/metabolism
MH  - Antibodies, Monoclonal/metabolism
MH  - Cell Line
MH  - Cell Proliferation
MH  - Centromere
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 13
MH  - Chromosomes, Human, Pair 7
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Trisomy
EDAT- 2008/01/30 09:00
MHDA- 2008/02/13 09:00
CRDT- 2008/01/30 09:00
PHST- 2008/01/30 09:00 [pubmed]
PHST- 2008/02/13 09:00 [medline]
PHST- 2008/01/30 09:00 [entrez]
PST - ppublish
SO  - Anal Quant Cytol Histol. 2007 Dec;29(6):351-7.