PMID- 18227722
OWN - NLM
STAT- MEDLINE
DCOM- 20080710
LR  - 20220309
IS  - 1541-2016 (Print)
IS  - 1533-4058 (Linking)
VI  - 16
IP  - 2
DP  - 2008 Mar
TI  - Digital image analysis improves the quality of subjective HER-2 expression 
      scoring in breast cancer.
PG  - 185-90
LID - 10.1097/PAI.0b013e318059c20c [doi]
AB  - AIM: To compare HER-2 scoring reproducibility by subjective and digital image 
      analysis (DIA) scores with each other and with fluorescence in situ hybridization 
      (FISH) assessed HER-2 amplification. METHODS: Herceptest-stained Tissue Micro 
      Arrays of 219 breast carcinomas were scored (DAKO protocol) by 3 observers (both 
      independent and as consensus), scored by DIA and both scores were compared with 
      FISH amplification results. RESULTS: Interobserver subjective scores 
      reproducibility was good (kappa 0.82 to 0.86) but therapeutically important 
      3+/2+discrepancies occurred in 11% to 16% of all 3+ cases. Subjective scores and 
      FISH results differed considerably. Consensus scores by 3 pathologists correlated 
      better with FISH, reducing the number of both Immunohistochemical (IHC) 
      negative/FISH positives and IHC 3+/FISH negatives. DIA scores were well 
      reproducible and correlated better with FISH amplification than did subjective 
      scores. CONCLUSIONS: DIA scores were comparable with consensus scores between 3 
      expert pathologists, were very well reproducible and performed better in 
      classifying IHC 3+/FISH+ cases than did subjective scores.
FAU - Skaland, Ivar
AU  - Skaland I
AD  - Department of Pathology, Stavanger University Hospital, Stavanger, The 
      Netherlands.
FAU - Ovestad, Irene
AU  - Ovestad I
FAU - Janssen, Emiel A M
AU  - Janssen EA
FAU - Klos, Jan
AU  - Klos J
FAU - Kjellevold, Kjell H
AU  - Kjellevold KH
FAU - Helliesen, Tove
AU  - Helliesen T
FAU - Baak, Jan P A
AU  - Baak JP
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Immunohistochem Mol Morphol
JT  - Applied immunohistochemistry & molecular morphology : AIMM
JID - 100888796
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Breast Neoplasms/*chemistry
MH  - Female
MH  - Genes, erbB-2
MH  - Humans
MH  - *Image Processing, Computer-Assisted
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Receptor, ErbB-2/*analysis
MH  - Reproducibility of Results
MH  - Tissue Array Analysis
EDAT- 2008/01/30 09:00
MHDA- 2008/07/11 09:00
CRDT- 2008/01/30 09:00
PHST- 2008/01/30 09:00 [pubmed]
PHST- 2008/07/11 09:00 [medline]
PHST- 2008/01/30 09:00 [entrez]
AID - 10.1097/PAI.0b013e318059c20c [doi]
PST - ppublish
SO  - Appl Immunohistochem Mol Morphol. 2008 Mar;16(2):185-90. doi: 
      10.1097/PAI.0b013e318059c20c.