PMID- 18228467
OWN - NLM
STAT- MEDLINE
DCOM- 20080320
LR  - 20161021
IS  - 1934-2616 (Electronic)
IS  - 1934-2616 (Linking)
VI  - Chapter 22
DP  - 2005 Jan
TI  - Comparative genomic hybridization.
PG  - Unit 22.6
LID - 10.1002/0471143030.cb2206s25 [doi]
AB  - Comparative genomic hybridization (CGH) is a screening method based on 
      fluorescence in situ hybridization (FISH). In contrast to conventional FISH, the 
      metaphase target is derived from a normal peripheral blood lymphocyte culture. 
      This target is hybridized to the test or tumor DNA, which is labeled/detected by 
      one fluorochrome (i.e., green), and to an equal amount of labeled normal or 
      reference DNA, which is labeled/detected by a different fluorochrome (red). It is 
      the difference in these green/red ratios (determined by specialized software) 
      along the length of each karyotyped chromosome that indicates the relative copy 
      number changes in the test/tumor DNA. The basic FISH techniques reviewed in this 
      section, the parameters for which also apply to obtaining satisfactory results 
      for CGH, include cytogenetic preparation and slide-making, DNA extraction (from 
      fresh or paraffin-embedded tissues) and labeling, slide pretreatment, 
      hybridization, post-hybridization washes, and detection.
FAU - Bayani, Jane
AU  - Bayani J
AD  - Princess Margaret Hospital and The Ontario Cancer Institute University of 
      Toronto, Toronto, Ontario, Canada.
FAU - Squire, Jeremy A
AU  - Squire JA
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Curr Protoc Cell Biol
JT  - Current protocols in cell biology
JID - 101287856
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Chromosome Painting/instrumentation/*methods
MH  - *Chromosomes, Human/genetics/metabolism/ultrastructure
MH  - DNA/*analysis/genetics
MH  - DNA Probes
MH  - DNA, Neoplasm/analysis/genetics
MH  - Genome, Human
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/instrumentation/*methods
MH  - Lymphocytes/physiology
MH  - Microscopy, Fluorescence/instrumentation/*methods
RF  - 12
EDAT- 2008/01/30 09:00
MHDA- 2008/03/21 09:00
CRDT- 2008/01/30 09:00
PHST- 2008/01/30 09:00 [pubmed]
PHST- 2008/03/21 09:00 [medline]
PHST- 2008/01/30 09:00 [entrez]
AID - 10.1002/0471143030.cb2206s25 [doi]
PST - ppublish
SO  - Curr Protoc Cell Biol. 2005 Jan;Chapter 22:Unit 22.6. doi: 
      10.1002/0471143030.cb2206s25.