PMID- 1823150
OWN - NLM
STAT- MEDLINE
DCOM- 19920922
LR  - 20121115
IS  - 0905-4383 (Print)
IS  - 0905-4383 (Linking)
VI  - 8
IP  - 6
DP  - 1991 Dec
TI  - The identification of photoallergic potential in the local lymph node assay.
PG  - 249-54
AB  - Guinea pig test methods are the most commonly used and reliable of predictive 
      models for contact photoallergenicity of chemicals. The murine local lymph node 
      assay (LLNA) has been developed recently as an alternative method for the 
      identification of skin-sensitizing chemicals. Sensitization potential is measured 
      from an assessment of the proliferation of lymphocytes in lymph nodes draining 
      the site of exposure to the test chemical. This work investigates the activity of 
      6 widely reported photoactive chemicals in a modified LLNA (a photo-LLNA). The 
      photoallergens tetrachlorosalicylanilide and fentichlor elicited positive 
      ultraviolet radiation (UV)-dependent proliferative responses that were greater 
      than their positive UV-independent responses, suggesting that they are both 
      contact and photoallergic in the mouse. The lack of a proliferative response to 
      6-methylcoumarin and the absence of a reproducible response to musk ambrette 
      suggest that the assay is insufficiently sensitive to identify weak photoallergic 
      potential. Positive UV-dependent responses to acridine and anthracene, both 
      photoirritants, cast doubt on the specificity of the photo-LLNA. Positive LLNA 
      responses to these chemicals may be due to skin protein modification, based on 
      evidence from the in vitro photo-chemical protein-binding assay. These results 
      demonstrate that the photo-LLNA is able to detect at least moderate photoallergic 
      potential.
FAU - Scholes, E W
AU  - Scholes EW
AD  - Environmental Safety Laboratory, Unilever Research and Engineering, Sharnbrook, 
      Bedford, United Kingdom.
FAU - Basketter, D A
AU  - Basketter DA
FAU - Lovell, W W
AU  - Lovell WW
FAU - Sarll, A E
AU  - Sarll AE
FAU - Pendlington, R U
AU  - Pendlington RU
LA  - eng
PT  - Journal Article
PL  - England
TA  - Photodermatol Photoimmunol Photomed
JT  - Photodermatology, photoimmunology & photomedicine
JID - 9013641
RN  - 0 (Acridines)
RN  - 0 (Allergens)
RN  - 0 (Anthracenes)
RN  - 0 (Chlorophenols)
RN  - 0 (Coumarins)
RN  - 0 (Dinitrobenzenes)
RN  - 0 (Salicylanilides)
RN  - 83-66-9 (musk ambrette (artificial))
RN  - 97-24-5 (fentichlor)
RN  - EH46A1TLD7 (anthracene)
RN  - EHP8W0L01A (6-methylcoumarin)
SB  - IM
MH  - Acridines/toxicity
MH  - *Allergens/drug effects
MH  - Animals
MH  - Anthracenes/toxicity
MH  - Cell Division
MH  - Chlorophenols/toxicity
MH  - Coumarins/toxicity
MH  - Dinitrobenzenes/toxicity
MH  - Lymph Nodes/drug effects/*pathology
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Photosensitivity Disorders/*diagnosis/pathology
MH  - Protein Binding
MH  - Salicylanilides/toxicity
EDAT- 1991/12/01 00:00
MHDA- 1991/12/01 00:01
CRDT- 1991/12/01 00:00
PHST- 1991/12/01 00:00 [pubmed]
PHST- 1991/12/01 00:01 [medline]
PHST- 1991/12/01 00:00 [entrez]
PST - ppublish
SO  - Photodermatol Photoimmunol Photomed. 1991 Dec;8(6):249-54.