PMID- 18234274
OWN - NLM
STAT- MEDLINE
DCOM- 20080805
LR  - 20220317
IS  - 0043-1354 (Print)
IS  - 0043-1354 (Linking)
VI  - 42
IP  - 8-9
DP  - 2008 Apr
TI  - Identification of important microbial populations in the mesophilic and 
      thermophilic phenol-degrading methanogenic consortia.
PG  - 1963-76
LID - 10.1016/j.watres.2007.11.037 [doi]
AB  - Active mesophilic and thermophilic phenol-degrading methanogenic consortia were 
      obtained after an 18-month acclimation and enriching process in the serum 
      bottles, and characterized using the rRNA-based molecular approach. As revealed 
      by cloning, fluorescence in situ hybridization (FISH) and terminal restriction 
      fragment length polymorphism (T-RFLP), these two enrichments differed greatly in 
      the community structures. The results for the first time suggest that group TA in 
      the Deltaproteobacteria (88.0% of EUBmix FISH-detectable bacterial cell area) and 
      Pelotomaculum spp. in the Desulfotomaculum family (81.2%) were the predominant 
      fermentative bacteria under mesophilic (37 degrees C) and thermophilic (55 
      degrees C) conditions, respectively. These populations closely associated with 
      mesophilic and thermophilic members of Methanosaetaceae, Methanobacteriaceae and 
      Methanomicrobiales to mineralize phenol as the sole carbon substrate to carbon 
      dioxide and methane. Moreover, these two enrichments could mineralize 
      terephthalate and benzoate. During benzoate degradation in the mesophilic 
      enrichment, a shift in the predominant bacterial population from 
      Deltaproteobacteria group TA to Syntrophus spp. was observed, suggesting 
      Syntrophus-related spp. could have a higher substrate affinity for benzoate. FISH 
      further revealed that member of the Deltaproteobacteria group TA represented more 
      than 68.3% of EUBmix FISH-detectable bacterial cell area in a full-scale 
      mesophilic bioreactor treating phenol-containing wastewaters.
FAU - Chen, Chia-Lung
AU  - Chen CL
AD  - Department of Civil Engineering, National University of Singapore, Singapore.
FAU - Wu, Jer-Horng
AU  - Wu JH
FAU - Liu, Wen-Tso
AU  - Liu WT
LA  - eng
PT  - Journal Article
DEP - 20071205
PL  - England
TA  - Water Res
JT  - Water research
JID - 0105072
RN  - 0 (DNA Primers)
RN  - 0 (Phenols)
RN  - 0 (RNA, Ribosomal)
SB  - IM
MH  - Base Sequence
MH  - DNA Primers
MH  - Euryarchaeota/genetics/*metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Phenols/*metabolism
MH  - RNA, Ribosomal/genetics
EDAT- 2008/02/01 09:00
MHDA- 2008/08/06 09:00
CRDT- 2008/02/01 09:00
PHST- 2007/05/16 00:00 [received]
PHST- 2007/11/22 00:00 [revised]
PHST- 2007/11/24 00:00 [accepted]
PHST- 2008/02/01 09:00 [pubmed]
PHST- 2008/08/06 09:00 [medline]
PHST- 2008/02/01 09:00 [entrez]
AID - S0043-1354(07)00723-3 [pii]
AID - 10.1016/j.watres.2007.11.037 [doi]
PST - ppublish
SO  - Water Res. 2008 Apr;42(8-9):1963-76. doi: 10.1016/j.watres.2007.11.037. Epub 2007 
      Dec 5.