PMID- 18236465
OWN - NLM
STAT- MEDLINE
DCOM- 20080522
LR  - 20220309
IS  - 0148-639X (Print)
IS  - 0148-639X (Linking)
VI  - 37
IP  - 4
DP  - 2008 Apr
TI  - Drug-screening platform based on the contractility of tissue-engineered muscle.
PG  - 438-47
LID - 10.1002/mus.20931 [doi]
AB  - A tissue-based approach to in vitro drug screening allows for determination of 
      the cumulative positive and negative effects of a drug at the tissue rather than 
      the cellular or subcellular level. Skeletal muscle myoblasts were 
      tissue-engineered into three-dimensional muscle with parallel myofibers 
      generating directed forces. When grown attached to two flexible micro-posts (mu 
      posts) acting as artificial tendons in a 96-well plate format, the miniature 
      bioartificial muscles (mBAMs) generated tetanic (active) forces upon electrical 
      stimulation measured with a novel image-based motion detection system. mBAM 
      myofiber hypertrophy and active force increased in response to insulin-like 
      growth factor 1. In contrast, mBAM deterioration and weakness was observed with a 
      cholesterol-lowering statin. The results described in this study demonstrate the 
      integration of tissue engineering and biomechanical testing into a single 
      platform for the screening of compounds affecting muscle strength.
FAU - Vandenburgh, Herman
AU  - Vandenburgh H
AD  - Department of Pathology, RISE Research Building, Brown Medical School/Miriam 
      Hospital, Providence, RI 02906, USA. herman_vandenburgh@brown.edu
FAU - Shansky, Janet
AU  - Shansky J
FAU - Benesch-Lee, Frank
AU  - Benesch-Lee F
FAU - Barbata, Victoria
AU  - Barbata V
FAU - Reid, Jonathan
AU  - Reid J
FAU - Thorrez, Lieven
AU  - Thorrez L
FAU - Valentini, Robert
AU  - Valentini R
FAU - Crawford, Gregory
AU  - Crawford G
LA  - eng
GR  - R41 AR 053386/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Muscle Nerve
JT  - Muscle & nerve
JID - 7803146
RN  - 0 (Heptanoic Acids)
RN  - 0 (Hydroxymethylglutaryl-CoA Reductase Inhibitors)
RN  - 0 (Pyrroles)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - A0JWA85V8F (Atorvastatin)
SB  - IM
MH  - Animals
MH  - Atorvastatin
MH  - Biological Assay/instrumentation/*methods
MH  - Cells, Cultured
MH  - Drug Evaluation, Preclinical/instrumentation/*methods
MH  - Electric Stimulation
MH  - Heptanoic Acids/pharmacology
MH  - Hydroxymethylglutaryl-CoA Reductase Inhibitors/pharmacology
MH  - Insulin-Like Growth Factor I/pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Muscle Contraction/*drug effects
MH  - Muscular Atrophy/*drug therapy/physiopathology
MH  - Myoblasts/cytology/drug effects
MH  - Pyrroles/pharmacology
MH  - Tissue Engineering/instrumentation/*methods
EDAT- 2008/02/01 09:00
MHDA- 2008/05/23 09:00
CRDT- 2008/02/01 09:00
PHST- 2008/02/01 09:00 [pubmed]
PHST- 2008/05/23 09:00 [medline]
PHST- 2008/02/01 09:00 [entrez]
AID - 10.1002/mus.20931 [doi]
PST - ppublish
SO  - Muscle Nerve. 2008 Apr;37(4):438-47. doi: 10.1002/mus.20931.