PMID- 18239176
OWN - NLM
STAT- MEDLINE
DCOM- 20080331
LR  - 20161122
IS  - 1524-4628 (Electronic)
IS  - 0039-2499 (Linking)
VI  - 39
IP  - 3
DP  - 2008 Mar
TI  - Brain-derived neurotrophic factor but not forced arm use improves long-term 
      outcome after photothrombotic stroke and transiently upregulates binding 
      densities of excitatory glutamate receptors in the rat brain.
PG  - 1012-21
LID - 10.1161/STROKEAHA.107.495069 [doi]
AB  - BACKGROUND AND PURPOSE: Both application of neurotrophic factors like 
      brain-derived neurotrophic factor (BDNF) and constraint-induced movement therapy 
      like forced arm use have been shown to potentially improve outcome after stroke. 
      The aim of the present study was to check whether postischemic long-term outcome 
      correlates to specific modifications in the abundance of various neurotransmitter 
      receptors. METHODS: Adult male Wistar rats were subjected to photothrombotic 
      ischemia and assigned to various treatment groups (n=5 each) with end points at 3 
      and 6 weeks: (1) ischemic control (saline); (2) BDNF (ischemia, 20 microg BDNF); 
      (3) forced arm use (ischemia, saline, and ipsilateral plaster cast for 5 or 14 
      days for the 3- and 6-week groups, respectively); and (4) combined treatment 
      (combi; ischemia, 20 microg BDNF, forced arm use). Animals received intravenous 
      bolus infusions of saline or BDNF 1 hour 3 and 5 days after ischemia, 
      respectively. A group of sham rats (n=2) served as a control. A battery of 
      behavioral tests was performed before and up to 6 weeks after ischemia. 
      Quantitative in vitro receptor autoradiography was performed on 12-microm-thick 
      cryostat sections using [(3)H]MK-801, [(3)H]AMPA, and [(3)H]muscimol for labeling 
      of NMDA, AMPA, and GABA(A) receptors, respectively. RESULTS: Best functional 
      outcome was seen after BDNF treatment, whereas vice versa rats with forced arm 
      use did worse in behavioral performance. Improved behavioral outcome was 
      associated with increased perilesional binding densities of NMDA and AMPA 
      receptors 3 weeks after stroke. CONCLUSIONS: Our findings suggest that transient 
      enhanced neurotransmission as reflected by increased ligand binding of NMDA and 
      AMPA receptors may participate in successful postlesional reorganization 
      processes.
FAU - Muller, Harald D
AU  - Muller HD
AD  - Department of Neuropathology, Johannes Gutenberg-University of Mainz, Mainz, 
      Germany.
FAU - Hanumanthiah, Kiran M
AU  - Hanumanthiah KM
FAU - Diederich, Kai
AU  - Diederich K
FAU - Schwab, Stefan
AU  - Schwab S
FAU - Schabitz, Wolf-Rudiger
AU  - Schabitz WR
FAU - Sommer, Clemens
AU  - Sommer C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080131
PL  - United States
TA  - Stroke
JT  - Stroke
JID - 0235266
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (GABA Agonists)
RN  - 0 (Ligands)
RN  - 0 (Receptors, Glutamate)
RN  - 2763-96-4 (Muscimol)
RN  - 6LR8C1B66Q (Dizocilpine Maleate)
RN  - 77521-29-0 (alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid)
SB  - IM
MH  - Animals
MH  - Autoradiography
MH  - Brain/*metabolism/pathology
MH  - Brain-Derived Neurotrophic Factor/*therapeutic use
MH  - Cerebral Infarction/etiology/pathology
MH  - Dizocilpine Maleate/metabolism
MH  - Excitatory Amino Acid Antagonists/metabolism
MH  - *Forelimb
MH  - GABA Agonists/metabolism
MH  - Intracranial Thrombosis/complications
MH  - Ligands
MH  - Male
MH  - Motor Activity/drug effects
MH  - Muscimol/metabolism
MH  - *Physical Therapy Modalities
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Glutamate/*metabolism
MH  - Restraint, Physical
MH  - Stroke/etiology/physiopathology/*therapy
MH  - Up-Regulation
MH  - alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid/metabolism
EDAT- 2008/02/02 09:00
MHDA- 2008/04/01 09:00
CRDT- 2008/02/02 09:00
PHST- 2008/02/02 09:00 [pubmed]
PHST- 2008/04/01 09:00 [medline]
PHST- 2008/02/02 09:00 [entrez]
AID - STROKEAHA.107.495069 [pii]
AID - 10.1161/STROKEAHA.107.495069 [doi]
PST - ppublish
SO  - Stroke. 2008 Mar;39(3):1012-21. doi: 10.1161/STROKEAHA.107.495069. Epub 2008 Jan 
      31.