PMID- 18241247
OWN - NLM
STAT- MEDLINE
DCOM- 20080522
LR  - 20080410
IS  - 1464-410X (Electronic)
IS  - 1464-4096 (Linking)
VI  - 101
IP  - 9
DP  - 2008 May
TI  - Messenger RNA expression of monocyte chemoattractant protein-1 and interleukin-6 
      in stone-containing kidneys.
PG  - 1170-7
LID - 10.1111/j.1464-410X.2008.07461.x [doi]
AB  - OBJECTIVES: To investigate the intrarenal mRNA expression of monocyte 
      chemoattractant protein-1 (MCP-1) and interleukin-6 (IL-6) in patients with 
      nephrolithiasis, and to evaluate whether their expression is associated with 
      renal function, as oxidative stress and inflammation are involved in the 
      pathogenesis of nephrolithiasis. PATIENTS, SUBJECTS AND METHODS: Renal biopsies 
      from near the stone, and blood and 24-h urine specimens were collected from 29 
      patients with nephrolithiasis. Control renal tissues were taken from 
      non-cancerous and cancerous portions of nephrectomy from six patients with renal 
      cancers, and control 24-h urine samples were obtained from 30 healthy subjects. 
      Corrected creatinine clearance, urinary N-acetyl-beta-glucosaminidase activity 
      and 8-hydroxy-deoxyguanosine (8-OHdG) were determined. The mRNA expressions of 
      MCP-1 and IL-6 in the tissues were measured by real time reverse 
      transcription-polymerase chain reaction. RESULTS: Patients with nephrolithiasis 
      had significantly greater renal tubular damage and oxidative stress than the 
      healthy controls. Intrarenal mRNA expressions of MCP-1 and IL-6 in stone-adjacent 
      renal tissues were significantly lower than in cancerous renal tissues, but not 
      statistically different from that in non-cancerous renal tissues. In 
      stone-adjacent renal tissues, the mRNA level of MCP-1 was significantly higher 
      than that of IL-6, but their expressions were significantly correlated with each 
      other. Histological examination showed that the number of infiltrated leukocytes 
      corresponded well with the intrarenal mRNA levels of MCP-1 and IL-6. Patients 
      with nephrolithiasis and compromised renal function had significantly higher 
      intrarenal mRNA levels of MCP-1 and IL-6 than those with preserved renal 
      function. Also, the mRNA levels in patients with severe renal tubular damage were 
      significantly greater than in those with less renal tubular damage. There was no 
      association between intrarenal mRNA expression and urinary 8-OHdG. CONCLUSION: 
      Nephrolithiasis was associated with low-grade intrarenal inflammation. A greater 
      intrarenal mRNA expression of MCP-1 and IL-6 was associated with enhanced renal 
      impairment. Thus, expression of MCP-1 and IL-6, at least in part, contributed to 
      the progression of nephrolithiasis.
FAU - Boonla, Chanchai
AU  - Boonla C
AD  - Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, 
      Bangkok, Thailand.
FAU - Hunapathed, Chanutra
AU  - Hunapathed C
FAU - Bovornpadungkitti, Sombat
AU  - Bovornpadungkitti S
FAU - Poonpirome, Kanitta
AU  - Poonpirome K
FAU - Tungsanga, Kriang
AU  - Tungsanga K
FAU - Sampatanukul, Pichet
AU  - Sampatanukul P
FAU - Tosukhowong, Piyaratana
AU  - Tosukhowong P
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
DEP - 20080130
PL  - England
TA  - BJU Int
JT  - BJU international
JID - 100886721
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Aged
MH  - Case-Control Studies
MH  - Chemokine CCL2/*metabolism
MH  - Female
MH  - Humans
MH  - Interleukin-6/*metabolism
MH  - Kidney/*pathology
MH  - Kidney Neoplasms/pathology
MH  - Male
MH  - Middle Aged
MH  - Nephritis/pathology
MH  - Nephrolithiasis/*pathology
MH  - Oxidative Stress/physiology
MH  - RNA, Messenger/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2008/02/05 09:00
MHDA- 2008/05/23 09:00
CRDT- 2008/02/05 09:00
PHST- 2008/02/05 09:00 [pubmed]
PHST- 2008/05/23 09:00 [medline]
PHST- 2008/02/05 09:00 [entrez]
AID - BJU7461 [pii]
AID - 10.1111/j.1464-410X.2008.07461.x [doi]
PST - ppublish
SO  - BJU Int. 2008 May;101(9):1170-7. doi: 10.1111/j.1464-410X.2008.07461.x. Epub 2008 
      Jan 30.