PMID- 18242016
OWN - NLM
STAT- MEDLINE
DCOM- 20080429
LR  - 20080225
IS  - 0378-4274 (Print)
IS  - 0378-4274 (Linking)
VI  - 177
IP  - 1
DP  - 2008 Feb 28
TI  - Analysis of differential gene expression in auricular lymph nodes draining skin 
      exposed to sensitizers and irritants.
PG  - 1-9
LID - 10.1016/j.toxlet.2007.12.003 [doi]
AB  - There has been some concern that certain non-sensitizing irritants may yield 
      false positive results in the murine local lymph node assay (LLNA). This study 
      compared gene expression profiles in lymph nodes draining skin following exposure 
      to sensitizers and irritants, to identify gene transcripts that could distinguish 
      sensitizers from irritants. After treating CBA/N mouse ears for 3 days with the 
      sensitizers 1-chloro-2,4-dinitrobenzene, 2-phenyl-4-ethoxymethylene-5-oxazolone, 
      or toluene-2,4-diisocyanate or the non-sensitizing irritants croton oil or 
      nonanoic acid, auricular lymph nodes and ear tissues were excised. 
      Sensitizer-induced changes in parameters such as ear thickness, lymph node 
      weight, and cell count also occurred in irritant-treated mouse tissues. However, 
      gene transcripts such as Ifi27, Il12rb1, Ifng, and Zbp1, which are related to 
      T-cell activation, were shown by gene expression microarrays and real-time RT-PCR 
      analyses to be up-regulated in auricular lymph nodes by sensitizers exclusively. 
      These findings suggest that gene expression analysis may enable distinction 
      between sensitizing chemicals and non-sensitizing irritants.
FAU - Ku, Hyun-Ok
AU  - Ku HO
AD  - Toxicology and Chemistry Division, National Veterinary Research and Quarantine 
      Service, Anyang, Republic of Korea.
FAU - Jeong, Sang-Hee
AU  - Jeong SH
FAU - Kang, Hwan-Goo
AU  - Kang HG
FAU - Pyo, Hyun-Mi
AU  - Pyo HM
FAU - Cho, Joon-Hyoung
AU  - Cho JH
FAU - Son, Seong-Wan
AU  - Son SW
FAU - Ryu, Doug-Young
AU  - Ryu DY
LA  - eng
PT  - Journal Article
DEP - 20080201
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Allergens)
RN  - 0 (Irritants)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Allergens/classification/*toxicity
MH  - Animals
MH  - Ear Auricle/*drug effects/metabolism/pathology
MH  - Female
MH  - Gene Expression/*drug effects/genetics
MH  - Gene Expression Profiling
MH  - Irritants/classification/*toxicity
MH  - Local Lymph Node Assay
MH  - Lymph Nodes/*drug effects/pathology
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Oligonucleotide Array Sequence Analysis
MH  - Organ Size/drug effects
MH  - RNA, Messenger/metabolism
MH  - Skin/*drug effects/metabolism/pathology
MH  - Up-Regulation/drug effects/genetics
EDAT- 2008/02/05 09:00
MHDA- 2008/04/30 09:00
CRDT- 2008/02/05 09:00
PHST- 2007/10/18 00:00 [received]
PHST- 2007/12/07 00:00 [revised]
PHST- 2007/12/07 00:00 [accepted]
PHST- 2008/02/05 09:00 [pubmed]
PHST- 2008/04/30 09:00 [medline]
PHST- 2008/02/05 09:00 [entrez]
AID - S0378-4274(07)01029-6 [pii]
AID - 10.1016/j.toxlet.2007.12.003 [doi]
PST - ppublish
SO  - Toxicol Lett. 2008 Feb 28;177(1):1-9. doi: 10.1016/j.toxlet.2007.12.003. Epub 
      2008 Feb 1.