PMID- 18242714 OWN - NLM STAT- MEDLINE DCOM- 20080623 LR - 20211020 IS - 0165-0270 (Print) IS - 0165-0270 (Linking) VI - 169 IP - 1 DP - 2008 Mar 30 TI - The actions of BDNF on dendritic spine density and morphology in organotypic slice cultures depend on the presence of serum in culture media. PG - 182-90 LID - 10.1016/j.jneumeth.2007.12.006 [doi] AB - We have previously shown that brain-derived neurotrophin factor (BDNF) increases dendritic spine density and the proportion of stubby spines in apical dendrites of CA1 pyramidal neurons of hippocampal slice cultures maintained in serum-free media. We show here that serum withdrawal causes an increase in the proportion of thin spines and a decrease in the fraction of stubby spines, without changing the overall density of dendritic spines. When slices are maintained in serum-containing media, BDNF also increased spine density but had the opposite effect on spine morphology: it increased the proportion of mushroom and thin spines and decreased the proportion of stubby spines. Intriguingly, slices maintained in serum media showed a lower p75NTR-to-TrkB expression level than serum-free slices, even after BDNF exposure. The differential actions of BDNF on spine morphology depending on the presence of serum in culture media, together with the difference in neurotrophin receptor expression are reminiscent of opposing functional signaling by p75NTR and Trk receptors, and reveal a complex modulation of dendritic morphology by BDNF signaling. FAU - Chapleau, Christopher A AU - Chapleau CA AD - Department of Neurobiology, Civitan International Research Center, McKnight Brain Institute, The University of Alabama at Birmingham, Birmingham, AL 35294-2182, USA. FAU - Carlo, Maria E AU - Carlo ME FAU - Larimore, Jennifer L AU - Larimore JL FAU - Pozzo-Miller, Lucas AU - Pozzo-Miller L LA - eng GR - P30 NS047466/NS/NINDS NIH HHS/United States GR - R21 NS057780-02/NS/NINDS NIH HHS/United States GR - P30 NS057098/NS/NINDS NIH HHS/United States GR - P30 HD038985/HD/NICHD NIH HHS/United States GR - R01 NS040593/NS/NINDS NIH HHS/United States GR - P30 NS047466-02/NS/NINDS NIH HHS/United States GR - P30 HD038985-059005/HD/NICHD NIH HHS/United States GR - R21 NS057780/NS/NINDS NIH HHS/United States GR - R01 NS040593-08/NS/NINDS NIH HHS/United States GR - PS30-NS47466/NS/NINDS NIH HHS/United States GR - P30-HD38985/HD/NICHD NIH HHS/United States GR - R01-NS40593/NS/NINDS NIH HHS/United States GR - P30-NS57098/NS/NINDS NIH HHS/United States GR - P30 NS057098-02/NS/NINDS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20071223 PL - Netherlands TA - J Neurosci Methods JT - Journal of neuroscience methods JID - 7905558 RN - 0 (Blood Proteins) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Culture Media) RN - 0 (Receptor, Nerve Growth Factor) RN - EC 2.7.10.1 (Receptor, trkB) SB - IM MH - Animals MH - Blood Proteins/*pharmacology MH - Brain-Derived Neurotrophic Factor/metabolism/*pharmacology MH - Cell Differentiation/drug effects/physiology MH - Cell Shape/drug effects/physiology MH - Culture Media/*pharmacology MH - Dendritic Spines/*drug effects/metabolism/ultrastructure MH - Hippocampus/cytology/*drug effects/metabolism MH - Microscopy, Confocal MH - Organ Culture Techniques/methods MH - Rats MH - Rats, Sprague-Dawley MH - Receptor, Nerve Growth Factor/drug effects/metabolism MH - Receptor, trkB/drug effects/metabolism MH - Signal Transduction/drug effects/physiology PMC - PMC2348185 MID - NIHMS43130 EDAT- 2008/02/05 09:00 MHDA- 2008/06/24 09:00 PMCR- 2009/03/30 CRDT- 2008/02/05 09:00 PHST- 2007/11/27 00:00 [received] PHST- 2007/12/11 00:00 [revised] PHST- 2007/12/11 00:00 [accepted] PHST- 2008/02/05 09:00 [pubmed] PHST- 2008/06/24 09:00 [medline] PHST- 2008/02/05 09:00 [entrez] PHST- 2009/03/30 00:00 [pmc-release] AID - S0165-0270(07)00609-7 [pii] AID - 10.1016/j.jneumeth.2007.12.006 [doi] PST - ppublish SO - J Neurosci Methods. 2008 Mar 30;169(1):182-90. doi: 10.1016/j.jneumeth.2007.12.006. Epub 2007 Dec 23.